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. 2019 Feb 15:474:67-71.
doi: 10.1016/j.carres.2019.01.007. Epub 2019 Jan 21.

Structure elucidation and gene cluster characterization of the O-antigen of Vibrio cholerae O14

Andrei V Perepelov  1 , Xiaodan Li  2 , Cong Xu  2 , Andrei V Filatov  3 , Alexander S Shashkov  3 , Sofia N Senchenkova  3 , Bin Liu  2
Affiliations

Structure elucidation and gene cluster characterization of the O-antigen of Vibrio cholerae O14

Andrei V Perepelov et al. Carbohydr Res. .

Abstract

The O-polysaccharide (O-antigen) of Vibrio cholerae O14 was studied using chemical analyses and 1D and 2D NMR spectroscopy. The following structure of the repeating unit of the O-antigen was established: where GlcpN(SHb) indicates 2-deoxy-2-[(S)-3-hydroxybutanoylamino]-d-glucose. We found that Vibrio cholerae O14 is similar to that of O-polysaccharide of Azospirillum brasilense S17, which has been reported earlier. Moreover, we predicted functions of all the genes in the O-antigen gene cluster according to the structure established. Our study enriches the existing O-antigen database of Vibrio cholerae, and further facilitates the bacterial serotype identification.

Keywords: Bacterial polysaccharide structure; GRASS; Lipopolysaccharide; O-antigen; O-antigen gene cluster; O-specific polysaccharide; Vibrio cholerae.

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