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. 2018 Winter;11(Suppl 1):S53-S58.

Apoptosis markers of circulating leukocytes are associated with the clinical course of inflammatory bowel disease

Azade Amini Kadijani  1 , Fariba Javadinia  2 , Alireza Mirzaei  3 , Zeinab Khazaei Koohpar  4 , Hedieh Balaii  2 , Shaghayegh Baradaran Ghavami  2 , Zahra Gholamrezaei  1 , Hamid Asadzadeh-Aghdaei  2
Affiliations

Apoptosis markers of circulating leukocytes are associated with the clinical course of inflammatory bowel disease

Azade Amini Kadijani et al. Gastroenterol Hepatol Bed Bench. 2018 Winter.

Abstract

Aim: Here we aimed at evaluating whether the apoptosis status of circulating leukocytes of inflammatory bowel diseases (IBD) patients is attributed to the diseases clinical status.

Background: Defects in the programmed cell death of inflammatory cells is known as to play a major role in the pathogenesis of IBD, and has been associated with the clinical efficacy of therapeutic agents.

Methods: A total of 50 IBD patients, 25 with remission and 25 with flare-up phase of the disease, who their disease was confirmed by colonoscopy, were included in this cross-sectional study. Pro-apoptotic Bax and anti-apoptotic Bcl-2 mRNA expression, along with Bax/Bcl-2 ratio, as measures of apoptotic status, were assessed in the Peripheral blood mononuclear cell (PBMC) of the patients using semi-quantitative Real-time PCR method.

Results: The mean Bax mRNA expression level was 0.54±0.12 in flare-up group and 0.53±0.13 in remission group (p=0.8). The mean Bcl-2 mRNA expression level was 0.63±0.13 in flare-up group and 0.55±0.12 in remission group (p=0.03). The mean Bax/Bcl-2 ratio was 0.88±0.17 in flare-up group and 1±21 in remission group (p=0.05). The mean Bax/Bcl-2 ratio was not statistically significant between different disease types (p=0.54) or therapeutic agents (p=0.7).

Conclusion: According to our results, alteration in markers of apoptosis could be traced in the circulating leukocytes of IBD patients, which suggest a potential for clinical application of apoptosis markers in disease monitoring and prediction of relapse.

Keywords: Apoptosis; Biomarker.; Flare-up; Inflammatory bowel diseases; Remission.

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Figures

Figure 1
Figure 1
Melting curves of Real-time PCR product for (A) Bax and (B) Bcl-2 gene
Figure 2
Figure 2
(A) Real time PCR analysis showing the mean Bax, Bcl-2, and Bax/Bcl-2 mRNA expression levels; (B) Comparison of mean Bax mRNA expression between remission and flare-up phases of IBD; (C) Comparison of mean Bcl-2 mRNA expression between remission and flare-up phases of IBD; (D) Comparison of mean Bax/Bcl-2 mRNA expression between remission and flare-up phases of IBD; Data are shown as mean±SD. (*) shows a significant p value (<0.05)
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