Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2019 May;15(5):258-260.
doi: 10.1038/s41581-019-0125-8.

Modelling diabetic vasculopathy with human vessel organoids

Ryuji Morizane  1   2   3   4
Affiliations
Comment

Modelling diabetic vasculopathy with human vessel organoids

Ryuji Morizane. Nat Rev Nephrol. 2019 May.

Abstract

A new study reports that human blood vessel organoids can be generated through the directed differentiation of human pluripotent stem cells. Use of these blood vessel organoids to model diabetic vasculopathy led to the identification of a new potential therapeutic target, suggesting this system could have translational value for studies of diabetes complications.

PubMed Disclaimer

Conflict of interest statement

Competing interests

R.M. is a co-inventor on patents (PCT/US16/52350) relating to organoid technologies that are assigned to Partners Healthcare.

Figures

Figure 1.
Figure 1.
Protocol for the generation of human blood vessel organoids to model diabetic vasculopathy. The directed differentiation of human pluripotent stem cells into human blood vessel organoids involves the treatment of stem cell aggregates in suspension culture with CHIR99021 (CHIR) to induce mesoderm differentiation. Vascular induction is then promoted through the addition of bone morphogenetic protein (BMP), vascular endothelial growth factor A (VEGF-A), and fibroblast growth factor 2 (FGF2), after which cell aggregates are embedded in a Matrigel–collagen I gel to facilitate the formation of a vascular network. Individual cell aggregates are then extracted from the gels and further differentiated with fetal bovine serum (FBS), VEGF-A and FGF2 in suspension culture, and the resulting organoids transplanted into diabetic mice.
See this image and copyright information in PMC

Comment on

  • Human blood vessel organoids as a model of diabetic vasculopathy.
    Wimmer RA, Leopoldi A, Aichinger M, Wick N, Hantusch B, Novatchkova M, Taubenschmid J, Hämmerle M, Esk C, Bagley JA, Lindenhofer D, Chen G, Boehm M, Agu CA, Yang F, Fu B, Zuber J, Knoblich JA, Kerjaschki D, Penninger JM. Wimmer RA, et al. Nature. 2019 Jan;565(7740):505-510. doi: 10.1038/s41586-018-0858-8. Epub 2019 Jan 16. Nature. 2019. PMID: 30651639 Free PMC article.

References

    1. Fatehullah A, Tan SH & Barker N Organoids as an in vitro model of human development and disease. Nature cell biology 18, 246–254, doi:10.1038/ncb3312 (2016). - DOI - PubMed
    1. Sato T et al. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature 459, 262–265, doi:10.1038/nature07935 (2009). - DOI - PubMed
    1. Morizane R & Bonventre JV Kidney Organoids: A Translational Journey. Trends in molecular medicine 23, 246–263, doi:10.1016/j.molmed.2017.01.001 (2017). - DOI - PMC - PubMed
    1. Morizane R et al. Nephron organoids derived from human pluripotent stem cells model kidney development and injury. Nature biotechnology 33, 1193–1200, doi:10.1038/nbt.3392 (2015). - DOI - PMC - PubMed
    1. Wimmer RA et al. Human blood vessel organoids as a model of diabetic vasculopathy. Nature 565, 505–510, doi:10.1038/s41586-018-0858-8 (2019). - DOI - PMC - PubMed