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. 2019 Feb 15;20(4):842.
doi: 10.3390/ijms20040842.

Osseointegration of Alkali-Modified NANOZR Implants: An In Vivo Study

Satoshi Komasa  1 , Mariko Nishizaki  2 , Honghao Zhang  3 , Seiji Takao  4 , Derong Yin  5 , Chisato Terada  6 , Yasuyuki Kobayashi  7 , Tetsuji Kusumoto  8 , Shigeki Yoshimine  9 , Hiroshi Nishizaki  10 , Joji Okazaki  11 , Luyuan Chen  12
Affiliations

Osseointegration of Alkali-Modified NANOZR Implants: An In Vivo Study

Satoshi Komasa et al. Int J Mol Sci. .

Abstract

Ingredients and surface modification methods are being continually developed to improve osseointegration of dental implants and reduce healing times. In this study, we demonstrate in vitro that, by applying concentrated alkali treatment to NANOZR with strong bending strength and fracture toughness, a significant improvement in the bone differentiation of rat bone marrow cells can be achieved. We investigated the influence of materials modified with this treatment in vivo, on implanted surrounding tissues using polychrome sequential fluorescent labeling and micro-computer tomography scanning. NANOZR implant screws in the alkali-treated group and the untreated group were evaluated after implantation in the femur of Sprague⁻Dawley male rats, indicating that the amount of new bone in the alkali-modified NANOZR was higher than that of unmodified NANOZR. Alkali-modified NANOZR implants proved to be useful for the creation of new implant materials.

Keywords: NANOZR; alkali treatment; in vivo study.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
SEM micrographs of control and test groups.
Figure 2
Figure 2
Scanning probe (SP) micrographs of control and test groups.
Figure 3
Figure 3
C1s, O1s, Zr3d XPS spectrum of the NANOZR surface (red line: control; blue line: alkali-treated NANOZR).
Figure 4
Figure 4
Micro-CT images (the implants are marked with red, cortical bone with blue, and cancellous bone with green).
Figure 5
Figure 5
Quantitative evaluation of the trabecular bone within ROI (BV/TV, Tb.N, Tb.Th, and Tb. Sp). * p < 0.05.
Figure 6
Figure 6
The longitudinally undecalcified histological sections with implants and peri-implant bones (purple: osteoid; white: new bone).
Figure 7
Figure 7
Quantitative histomorphometric analysis within the measured region (bone-implant contact (BIC) and bone area ratio (BA)). * p < 0.05.
Figure 8
Figure 8
The histological sections were also observed using confocal laser scanning microscopy for dynamic histomorphometry according to fluorescence labeling (blue: 1 week of new bone, yellow: 4 week of new bone, green: 8 week of new bone, red: osteoid).
Figure 9
Figure 9
The percentage of labeled bone area (%LBA). * p < 0.05.
Figure 10
Figure 10
The control and alkali-treated NANOZR implant photographs.
Figure 11
Figure 11
Surgical procedure.
See this image and copyright information in PMC

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