Circular RNA expression in exosomes derived from breast cancer cells and patients

Abstract

Aim: We aimed to explore the roles of circular RNAs (circRNAs) in breast cancer (BCa).

Materials & methods: RNA was extracted from exosomes and BCa cells and analyzed using the RNA sequencing technique or microarray.

Results: Compared with controls, 1147 and 1195 circRNAs were dysregulated in exosomes from metastatic and localized BCa patients, respectively. A total of 480 dysregulated circRNAs were found in metastatic patients compared with localized patients, and these dysregulated circRNAs were enriched in eight pathways. Compared with MCF-7 cells and their exosomes, there were 5842 and 1137 dysregulated circRNAs in MDA-MB-231 cells and exosomes, respectively, and 5 circRNAs were confirmed using real-time quantitative PCR.

Conclusion: We identified a number of dysregulated circRNAs in exosomes from BCa cells and patients.

Keywords: breast cancer; breast carcinoma; carcinogenesis; circular RNA; exosomes; extracellular vesicles; invasiveness; metastasis; miRNA; pathway.