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. 2018 Dec;10(4):222-225.
doi: 10.1556/1646.10.2018.42.

Mutans Streptococci counts from saliva and its protein profile in early childhood caries

Endang W Bachtiar  1   2 , Ferry P Gultom  1 , Atika Rahmasari  1 , Boy M Bachtiar  1   2
Affiliations

Mutans Streptococci counts from saliva and its protein profile in early childhood caries

Endang W Bachtiar et al. Interv Med Appl Sci. 2018 Dec.

Abstract

Aim: This study aims to analyze the number Mutans Streptococci (MS) and its protein profile from the saliva of early childhood caries (ECC) and caries-free subjects.

Methods: MS counts were cultured from saliva samples, and the protein profile of MS was determined from ECC and caries-free subjects. The number of colonies were counted, and the protein bands with the molecular weight of 13, 29, 39, 41.3, 74, and 95 kDa were determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis method.

Results: We found that the number of colonies from saliva of ECC patients was higher than those caries-free (22.20 × 106 CFU/ml vs. 19.16 × 106 CFU/ml, p < 0.05). There are higher expression frequencies in protein 29, 39, 41.3, and 74 kDa of MS in ECC than caries-free subjects.

Conclusions: There is the higher number of MS colonies and difference of MS protein profile isolated from saliva among children with ECC and caries-free counterparts.

Keywords: Mutans Streptococci; dmft; early childhood caries; protein profile; saliva.

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Figures

Fig. 1.
Fig. 1.
The quantity of Mutans Streptococci in saliva of ECC patients and caries-free children
Fig. 2.
Fig. 2.
Distribution of the quantity of Mutans Streptococci in saliva of low, moderate, and high dmft of patients. *p < 0.05
Fig. 3.
Fig. 3.
The expression of protein with molecular weight of Mutans Streptococci in ECC and caries-free subjects
See this image and copyright information in PMC

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