Slow Dynamics in Microcolumnar Gap Junction Network of Developing Neocortical Pyramidal Neurons

Abstract

Gap junctions mediate electrical coupling between neurons and modulate their firing activity. In mouse neocortical layer 5, the major types of pyramidal neurons organize into cell type-specific microcolumns that exhibit modular neuronal activity. During cortical development, microcolumn neurons are electrically coupled in a cell type-specific manner at the stage of synaptogenesis, forming a dense network of gap junctions. However, modulation of neuronal activity by the gap junction network has not been examined. Here, we show that the electrical coupling induces amplification and slow synchronization of action potentials. This slow synchronization is mediated by electrical transmission that is an order of magnitude slower than that of gap junction-coupled neurons of other types. Theoretical and structural analyses suggested that apical dendrites are a major site of electrical coupling, providing slow electrical transmission. These results suggest that the gap junction network organizes neuronal activity of developing cortical circuit modules with unique slow dynamics.

Keywords: cerebral cortex; dendrites; gap junction; neocortex; pyramidal neuron.