Impact of heat stress on development and fertility of Drosophila suzukii Matsumura (Diptera: Drosophilidae)

Abstract

Drosophila suzukii is a globally invasive fruit pest that costs millions in yield losses and increased pest management costs. Management practices for D. suzukii currently rely heavily on calendar-based applications of broad-spectrum insecticides, but decision-based applications are theoretically possible with refined population modeling and monitoring. Temperature conditions are strongly deterministic of insect growth rates, fecundity, fertility, and resulting population densities. Therefore, information about the effects of temperature can be incorporated into population modeling to accurately predict D. suzukii population densities in the field which is crucial to maximize pesticide application efficiency and improve sustainability. Here, we investigated the effects of chronic heat stress during all of juvenile development on egg-to-adult viability and fertility. We also investigated egg-to-adult viability under heat stress after heat shock of the maternal parent. We found that heat stress during development results in lower egg-to-adult viability, and reduced lifespan and fertility for surviving adults. However, heat-shock treatment of females prior to egg laying increased the egg-to-adult viability of their eggs under heat stress. Female flies that developed at 30 °C had smaller ovaries than the untreated group and male flies had less sperm in their testes, and no sperm in their seminal vesicles. We conclude that heat stress during development is likely to have negative effect on D. suzukii population dynamics in the field. However, the intensity of such negative impact will depend on the phenotypic state of their maternal parents.

Keywords: Development; Drosophila; Fertility; Heat shock; Heat stress; Penotypic plasticity; Transgenerational.