Amperometric biosensors based on oxidases and PtRu nanoparticles as artificial peroxidase

Abstract

Catalytically active nanomaterials have several advantages over their natural analogues when used as artificial enzymes (nanozymes), namely, higher stability and lower cost. Nanozymes with metallic nanocomposites are promising catalysts for biosensing applications. The aim of the current research is to construct oxidase-based bioelectrodes for food analysis using nanozymes as peroxidase mimetics. Bimetallic PtRu nanoparticles (nPtRu) coupled with alcohol oxidase (AO) and methylamine oxidase (AMO) were chosen to construct amperometric biosensors (ABSs) for primary alcohols and methylamine (MA). Both ABSs show high sensitivities (336 A·M^-1·m^-2 for the AO-ABS and 284 A·M^-1·m^-2 for the AMO-ABS), broad linear ranges (25-200 µM ethanol and 20-600 µM MA) and satisfactory storage stabilities. Practical feasibility of the constructed ABSs was demonstrated on food samples. High correlation between contents of MA and ethanol in foods determined by the ABSs and reference methods was observed.

Keywords: Alcohol oxidase; Amperometric biosensor; Artificial peroxidase; Chloroplatinic acid (PubChem CID: 44134627); Dimethylamine (PubChem CID: 674); Ethanol (PubChem CID:702); Ethanol and methylamine analysis; Formaldehyde (PubChem CID: 62705); Glucose (PubChem CID: 107526); Hydrogen peroxide (PubChem CID: 784); Methanol (PubChem CID: 887); Methylamine (PubChem CID:6329); Methylamine oxidase; PtRu nanoparticles; Ruthenium trichloride hydrate (PubChem CID: 6093376); Trimethylamine (PubChem CID: 1146).