The energy cost of the tonoplast futile sodium leak

Abstract

Active removal of Na⁺ from the cytosol into the vacuole plays a critical role in salinity tissue tolerance, but another, often neglected component of this trait is Na⁺ retention in vacuoles. This retention is based on an efficient control of Na⁺ -permeable slow- and fast-vacuolar channels that mediate the back-leak of Na⁺ into cytosol and, if not regulated tightly, could result in a futile cycle. This Tansley insight summarizes our current knowledge of regulation of tonoplast Na⁺ -permeable channels and discusses the energy cost of vacuolar Na⁺ sequestration, under different scenarios. We also report on a phylogenetic and bioinformatic analysis of the plant two-pore channel family and the difference in its structure and regulation between halophytes and glycophytes, in the context of salinity tolerance.

Keywords: H+-ATPase; halophyte; salinity stress; tonoplast ion channels; two-pore channel 1 (TPC1); vacuolar sodium sequestration.