Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Jan-Feb;23(1):22-26.
doi: 10.1016/j.bjid.2019.01.005. Epub 2019 Feb 23.

Polyomaviruses BK and JC DNA infection in peripheral blood cells from blood donors

Malihe Fathi Haghighi  1 , Noorossadat Seyyedi  2 , Ali Farhadi  2 , Farahnaz Zare  2 , Leila Kasraian  3 , Gholam Reza Refiei Dehbidi  2 , Reza Ranjbaran  2 , Abbas Behzad-Behbahani  4
Affiliations

Polyomaviruses BK and JC DNA infection in peripheral blood cells from blood donors

Malihe Fathi Haghighi et al. Braz J Infect Dis. 2019 Jan-Feb.

Abstract

Objectives: To investigate the prevalence of human polyomavirus (BK and JC viruses) infection in peripheral blood mononuclear cells of healthy blood donors.

Methods: The study included 250 healthy blood donors. Five-milliliter blood was drawn into sterile EDTA tubes and PBMCs were isolated from whole blood. The isolated PBMCs were counted and stored at -70°C for future investigation. DNA was extracted and subjected to simple, sensitive and specific semi-nested PCR as well as QPCR using both general and specific primers for different assays.

Results: Of 250 blood samples, 66 (26.4%) were positive for BKV DNA (146-34,514 copies/106 cells). JC DNA was found in 45 (18%) blood samples (65-21,250 copies/106 cells). Co-infection with these viruses were found in 11 (4.4%) out of 250 blood samples.

Discussion: Our study provides important data on polyomavirus infection in peripheral blood mononuclear leukocytes in immunocompetent individuals. These data indicate significant differences between the prevalence of BKV and JCV infection in healthy blood donors. The prevalence of BK and JC virus infection is higher in the age range 30-39 years compared to other age ranges.

Keywords: Blood donors; Leukocytes; Mononuclear; Polyomavirus; Real-time polymerase chain reaction.

PubMed Disclaimer

References

    1. Khalili K., Stoner G.L. John Wiley & Sons; 2004. Human polyomaviruses: molecular and clinical perspectives.
    1. Arthur R.R., Dagostin S., Shah K.V. Detection of BK virus and JC virus in urine and brain tissue by the polymerase chain reaction. J Clin Microbiol. 1989;27:1174–1179. - PMC - PubMed
    1. Butel J.S., Lednicky J.A. Cell and molecular biology of simian virus 40: implications for human infections and disease. J Natl Cancer Inst. 1999;91:119–134. - PubMed
    1. Lednicky J.A., Arrington A.S., Stewart A.R., et al. Natural isolates of simian virus 40 from immunocompromised monkeys display extensive genetic heterogeneity: new implications for polyomavirus disease. J Virol. 1998;72:3980–3990. - PMC - PubMed
    1. Weber T., Major E.O. Progressive multifocal leukoencephalopathy: molecular biology, pathogenesis and clinical impact. Intervirology. 1997;40:98–111. - PubMed

MeSH terms