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. 2019 Feb 26;9(1):2743.
doi: 10.1038/s41598-019-39154-w.

Analysis of wheat gene expression related to the oxidative stress response and signal transduction under short-term osmotic stress

Karolina Dudziak  1 , Magdalena Zapalska  1 , Andreas Börner  2 , Hubert Szczerba  3 , Krzysztof Kowalczyk  1 , Michał Nowak  4
Affiliations

Analysis of wheat gene expression related to the oxidative stress response and signal transduction under short-term osmotic stress

Karolina Dudziak et al. Sci Rep. .

Abstract

Water shortage is a major environmental stress that causes the generation of reactive oxygen species (ROS). The increase in ROS production induces molecular responses, which are key factors in determining the level of plant tolerance to stresses, including drought. The aim of this study was to determine the expression levels of genes encoding MAPKs (MAPK3 and MAPK6), antioxidant enzymes (CAT, APX and GPX) and enzymes involved in proline biosynthesis (P5CS and P5CR) in Triticum aestivum L. seedlings in response to short-term drought conditions. A series of wheat intervarietal substitution lines (ISCSLs) obtained by the substitution of single chromosomes from a drought-sensitive cultivar into the genetic background of a drought-tolerant cultivar was used. This source material allowed the chromosomal localization of the genetic elements involved in the response to the analyzed stress factor (drought). The results indicated that the initial plant response to drought stress resulted notably in changes in the expression of MAPK6 and CAT and both the P5CS and P5CR genes. Our results showed that the substitution of chromosomes 3B, 5A, 7B and 7D had the greatest impact on the expression level of all tested genes, which indicates that they contain genetic elements that have a significant function in controlling tolerance to water deficits in the wheat genome.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
The pattern of MAPK3 (a) and MAPK6 (b) genes expression alteration in S29(JP) substitution lines after 1, 3 and 6 h of 10% PEG treatment. The average values obtained for all tested genotypes in particular time point are presented in comparison to non-exposed plants (Control). Bars represent standard deviation. All samples were analyzed in three full biological and three technical replications.
Figure 2
Figure 2
Changes in expression of MAPK3 (a) and MAPK6 (b) genes in S29(JP) substitution lines after 1, 3 and 6 h of 10% PEG treatment. Bars represent standard deviation. The expression level for non-exposed plants was used as calibrator (relative expression level = 1), *change significant at the 0.05 level. All samples were analyzed in three full biological and three technical replications.
Figure 3
Figure 3
The pattern of CAT (a), APX (b), and GPX (c) genes expression alteration in S29(JP) substitution lines after 1, 3 and 6 h of 10% PEG treatment. The average values obtained for all tested genotypes in particular time point are presented in comparison to non-exposed plants (Control). Bars represent standard deviation. All samples were analyzed in three full biological and three technical replications.
Figure 4
Figure 4
Changes in expression of CAT (a), APX (b), and GPX (c) genes in S29(JP) substitution lines after 1, 3 and 6 h of 10% PEG treatment. Bars represent standard deviation. The expression level for non-exposed plants was used as calibrator (relative expression level = 1), *change significant at the 0.05 level. All samples were analyzed in three full biological and three technical replications.
Figure 5
Figure 5
The pattern of P5CS (a) and P5CR (b) genes expression alteration in S29(JP) substitution lines after 1, 3 and 6 h of 10% PEG treatment. The average values obtained for all tested genotypes in particular time point are presented in comparison to non-exposed plants (Control). Bars represent standard deviation. All samples were analyzed in three full biological and three technical replications.
Figure 6
Figure 6
Changes in expression of P5CS (a) and P5CR (b) genes in S29(JP) substitution lines after 1, 3 and 6 h of 10% PEG treatment. Bars represent standard deviation. The expression level for non-exposed plants was used as calibrator (relative expression level = 1), *change significant at the 0.05 level. All samples were analyzed in three full biological and three technical replications.
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