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. 2019 Mar;37(3):224-226.
doi: 10.1038/s41587-019-0032-3.

CRISPResso2 provides accurate and rapid genome editing sequence analysis

Kendell Clement  1   2   3 , Holly Rees  4   5   6 , Matthew C Canver  1   2   3 , Jason M Gehrke  2   3 , Rick Farouni  1   2   3 , Jonathan Y Hsu  1   2   3 , Mitchel A Cole  7 , David R Liu  4   5   6 , J Keith Joung  2   3 , Daniel E Bauer  8   9   10   11 , Luca Pinello  12   13   14
Affiliations

CRISPResso2 provides accurate and rapid genome editing sequence analysis

Kendell Clement et al. Nat Biotechnol. 2019 Mar.
No abstract available

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Figures

Figure 1:
Figure 1:. Novel features of CRISPResso2.
a-c) CRISPResso2 analysis of base editing data. a) Locations of substitutions across the FANCF reference sequence for the BE3 base editor. At each position, the number of substitutions from the reference base to each non-reference base are shown. The quantification window is outlined by the dashed gray box. b) Barplot showing the frequency of substitution from a reference base to a non-reference base including only bases in the quantification window from part a. c) Batch output mode comparing the editing efficiencies of three base editors and an untreated control at the FANCF locus. C>T conversion rates are shown at each cytosine overlapping the guide. d) Allele-specific editing outcomes of SaCas9-KKH editing of the Rho gene in P23H heterozygous mice . Reads (left) can be assigned to each allele using CRISPResso2 (right) to achieve accurate quantification of genome editing at genomic loci with multiple alleles. The pie chart shows the assignment of each read to the wild-type (red and dark blue) allele or to the P23H allele (yellow and light blue). Ambiguous alignments that could not be attributed uniquely to one of the alleles (e.g., due to a deletion at the SNP location) are shown in purple.
See this image and copyright information in PMC

References

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