Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Jun 1;147(6):1843-8.
doi: 10.1084/jem.147.6.1843.

Hematopoietic thymocyte precursors: IV. Enrichment of the precursors and evidence for heterogeneity

R S Basch, J L Kadish, G Goldstein

Hematopoietic thymocyte precursors: IV. Enrichment of the precursors and evidence for heterogeneity

R S Basch et al. J Exp Med. .

Abstract

A method has been developed for the enrichment of the hematopoietic precursors of thymocytes from spleen and bone marrow cells. Up to 40-fold enrichments were obtained resulting in preparations in which as few as 10(5) cells produced prompt repopulation of the thymus of an irradiated mouse. Precursor cells from bone marrow appear to contain the enzyme terminal deoxyribonucleotidyl transferase (Tdt), an agent suggested as a potential somatic mutator. This enzyme (Tdt) was not detectable in any spleen cell preparation examined, including one in which a 40-fold enrichment of thymocyte precursors had been produced. This is the first difference reported between the splenic and bone marrow precursors of thymocytes.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Exp Med. 1977 Feb 1;145(2):405-19 - PubMed
    1. Nature. 1974 Mar 29;248(447):409-11 - PubMed
    1. Biochem Biophys Res Commun. 1971 Jul 2;44(1):124-31 - PubMed
    1. Nat New Biol. 1973 Sep 5;245(140):8-10 - PubMed
    1. Lancet. 1973 Apr 7;1(7806):740-3 - PubMed

Publication types

Substances