Evaluation of Tooth Surface Irradiated With Erbium: Yttrium Aluminum Garnet and Carbon Dioxide Lasers by Atomic Force Microscopy

Abstract

Introduction: Laser irradiation of dentin surface can affect its surface topography and roughness. Atomic force microscopy (AFM) is among the most efficient tools for determination of surface topography of natural biomolecules in nano-scales. Surface roughness affects plaque retention especially in the cervical region. This study aimed to assess and compare the obstruction of dentinal tubules and dentin surface roughness after irradiation of erbium: yttrium aluminum garnet (Er:YAG ) and CO2 lasers for treatment of dentin hypersensitivity (DH). Methods: Five disc-shaped samples measuring 3 mm in thickness were fabricated by horizontal sectioning of the cervical area of five extracted human molars using a low speed saw. Each disc was divided into 3 segments by a bur under water coolant. The three segments of each disc were placed on a glass slide. First segment: No intervention (control group). Second segment: Er:YAG laser irradiation (2940 nm, 50 mJ, 10 Hz and 0.5 W, 30 seconds) along with water coolant. Third segment: CO2 laser irradiation (10600 nm, 80 Hz, 0.3 W, 30 seconds) along with water coolant. After that, the surfaces underwent non-contact AFM. The diameters of dentinal tubules as well as surface roughness were then measured and statistically analyzed using repeated measures ANOVA. Results: The surface roughness parameters (Ra, Rq) showed increased roughness after laser irradiation and this increase in roughness after Er:YAG laser application was significant compared to the control group (P=0.048). However, CO2 laser caused no significant change in surface roughness. Also, after Er:YAG laser application, fewer open dentinal tubules were observed and the remaining open tubules had a smaller diameter. Conclusion: Based on the results, Er:YAG laser irradiation obstructs the dentinal tubules and increases the dentin surface roughness. This increase in surface roughness can cause microbial plaque retention and increase the risk of caries and periodontal disease. Application of CO2 laser (compared to Er:YAG) lead to slight but clinically significant obstruction of dentinal tubules and surface roughness.

Keywords: Atomic force microscopy.; Dental tubule; Dentin hypersensitivity; Laser.

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