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. 2019 Feb 18;12(2):189-193.
doi: 10.18240/ijo.2019.02.01. eCollection 2019.

Effect of topical 0.05% cyclosporine A on the tear protein lacritin in a rat model of dry eye

Fen-Dui Zhang  1 , Zhao-Qin Hao  2 , Wei Gao  2 , Yi-Qiao Xing  1
Affiliations

Effect of topical 0.05% cyclosporine A on the tear protein lacritin in a rat model of dry eye

Fen-Dui Zhang et al. Int J Ophthalmol. .

Abstract

Aim: To observe the effect of topical 0.05% cyclosporine A (CsA) on the ocular surface and tear protein lacritin in a botulinum B-induced dry eye rat model.

Methods: A total of 36 female SD rats were randomly divided into 3 groups, botulinum B was injected into the right lacrimal gland of all rats. Group A and group B were treated with 0.05% CsA and 0.1% sodium hyaluronate, respectively, 3 times daily. The control group was not treated. Basal tear flow, corneal epithelial defects, and lacritin levels were measured.

Results: Tear secretion in all rats was reduced on day 3 and was even lower on day 7 postoperation (P<0.05). Tear secretion in group A increased by day 14 and was at the preoperative level on day 42. Tear secretion in group B and control rats was lower on days 14 and 42 compared with preoperative level (P<0.05). Corneal fluorescein staining in group A was higher on day 3, peaked on day 7, and then decreased gradually from day 7 until day 14, returning to normal by day 42 post-procedure. However, in group B, corneal fluorescein staining had improved, but was not fully recovered by day 42. Corneal fluorescein staining was more intense than before the operation and then in the control group at all time points. Tear protein lacritin levels reached the lowest levels on day 7 in all groups. In group A, tear protein lacritin levels began to increase on day 14 and were normal on day 42. In group B, tear protein lacritin levels began to increase on day 14, but had not completely recovered on day 42. In the control group, tear protein lacritin levels remained low post-procedure.

Conclusion: CsA 0.05% prompts tear protein lacritin expression in a rat model of dry eye and improves the signs and symptoms of dry eye disease.

Keywords: 0.05% cyclosporine A; dry eye; tear protein lacritin.

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Figures

Figure 1
Figure 1. Tear protein lacritin expression
A: Lacrimal acinar cells (green fluorescein); B: Conjunctival surface (green fluorescence).
Figure 2
Figure 2. Fluctuation of tear protein lacritin levels of groups at all time points.
See this image and copyright information in PMC

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References

    1. McKown RL, Wang NN, Raab RW, Karnati R, Zhang YH, Williams PB, Laurie GW. Lacritin and other new proteins of the lacrimal functional unit. Exp Eye Res. 2009;88(5):848–858. - PMC - PubMed
    1. Moss SE. Prevalence of and risk factors for dry eye syndrome. Arch Ophthalmol. 2000;118(9):1264. - PubMed
    1. Ayaki M, Kawashima M, Uchino M, Tsubota K, Negishi K. Gender differences in adolescent dry eye disease: a health problem in girls. Int J Ophthalmol. 2018;11(2):301–307. - PMC - PubMed
    1. Xu L, Zhang W, Zhu XY, Suo T, Fan XQ, Fu Y. Smoking and the risk of dry eye: a Meta-analysis. Int J Ophthalmol. 2016;9(10):1480–1486. - PMC - PubMed
    1. You YS, Qu NB, Yu XN. Alcohol consumption and dry eye syndrome: a Meta-analysis. Int J Ophthalmol. 2016;9(10):1487–1492. - PMC - PubMed

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