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. 2018 Dec;4(4):12-19.
doi: 10.18502/cmm.4.4.381.

Genotyping of Candida albicans isolated from animals using 25S ribosomal DNA and ALT repeats polymorphism in repetitive sequence

Armina Dalvand  1 , Farzad Katiraee  1 , Raziallah Jafari Joozani  2 , Hojjatolah Shokri  3
Affiliations

Genotyping of Candida albicans isolated from animals using 25S ribosomal DNA and ALT repeats polymorphism in repetitive sequence

Armina Dalvand et al. Curr Med Mycol. 2018 Dec.

Abstract

Background and purpose: Candida albicans is the most prevalent Candida species isolated from animals. Candidiasis can be systemic in animals or may affect a single organ, such as the mouth, urinary tract, and skin. The aim of the present study was to determine the genetic diversity of C. albicans isolated from different animals and investigate the presence of a relationship between host specificity and genetic typing of C. albicans.

Materials and methods: For the purpose of the study, DNA extraction was performed on 27 clinical isolates of C. albicans obtained from animals. Subsequently, they were subjected to 25S ribosomal DNA amplification and ALT repeats in repetitive sequences (RPSs). The minimum inhibitory concentrations of fluconazole, ketoconazole, clotrimazole, nystatin, amphotericin B, and caspofungin were determined using the microdilution method based on the Clinical and Laboratory Standards Institute M27-S4 standard.

Results: Out of 27 C. albicans strains, 11, 6, 5, and 5 cases were recognized as genotypes A (40.8%), E (22.2%), B (18.5%), and C (18.5%), respectively, through amplification using AS-I, which revealed 17 different types of C. albicans. By combining the two typing methods, 27 C. albicans strains were finally divided into 22 genotypes.

Conclusion: Different genotypes showed genetic diversity among the C. albicans strains isolated from animal sources. The results revealed no special genotype relationship according to the host, anatomical source of isolation, and antifungal susceptibility.

Keywords: Animal; Antifungals; Candida albicans; Genotyping; Repetitive sequences.

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Figures

Figure 1
Figure 1
Amplification profiles of polymerase chain reaction (PCR) products of Candida albicans isolates in the PCRs targeting 25S rDNA (ABC typing) (Genomic DNA purified from various yeast species was amplified by PCR using CA-INT primer.)
Figure 2
Figure 2
Subtype analysis of Candida albicans by repetitive sequences (RPS) primers (Amplification profiles of polymerase chain reaction (PCR) products of C. albicans isolates in the PCRs targeting RPSs. Genomic DNA purified from various yeast species was amplified by PCR using AS-I primer.)
Figure 3
Figure 3
UPGMA dendrogram using the Dice similarity coefficient, genetic variation among Candida albicans strains based on 25S rDNA and ALT-based genotyping (22 genotypes were identified out of 27 C. albicans isolated from animals.)
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