Phylogeny and taxonomy of three new Ctenomyces (Arthrodermataceae, Onygenales) species from China

Abstract

Twelve Ctenomyces (Arthrodermataceae, Onygenales) strains were obtained and identified during a survey of keratinophilic fungi in soils from China. We used molecular identification combined with morphological evidence to delimit species, circumscribing five species in the genus. Three new species are herein described: C.albus sp. nov., C.obovatus sp. nov. and C.peltricolor sp. nov. We also described, illustrated and compared the novel species with related species in the morphology.

Keywords: Ctenomyces; 3 new species; Filamentous fungi; Morphology; Multigene.

Figure 1.

Phylogenetic tree of Arthrodermataceae based on the ITS dataset and Myceliophthoralutea (CBS 145.77 and MUCL 10070) as the outgroup taxon. Numbers at nodes are Bayesian posterior probabilities (left, BPP ≥0.75) and maximum likelihood bootstrap values (right, BS ≥70%).

Figure 2.

Phylogenetic tree of Ctenomyces based on the ITS+EF1A+RPB2 dataset and Myceliophthoralutea (CBS 145.77 and MUCL 10070) as the outgroup taxon. Numbers at nodes are Bayesian posterior probabilities (left, BPP ≥0.9) and maximum likelihood bootstrap values (right, ≥95%).

Figure 3.

Ctenomycesalbus (from ex-holotype strain CGMCC 3.19232). A–C Conidiogenous structures and conidia D, E Intercalary conidia F, G Colony on PDA at day 14. Scale bars: 10 µm (A–E); 10 mm (F, G).

Figure 4.

Ctenomycesobovatus (from ex-holotype strain CGMCC 3.19225). A–D Conidiogenous structures and conidia E, F Colony on PDA at day 14. Scale bars: 10 µm (A–D); 10 mm (E, F).

Figure 5.

Ctenomycespeltricolor (from ex-holotype strain CGMCC 3.19229) A–D Conidiogenous structures and conidia E, F Colony on PDA at day 14. Scale bars: 10 µm (A–D); 10 mm (E, F).

Figure 6.

Ctenomycesserratus (from strain CGMCC 3.18622) A–E Conidiogenous structures and conidia F, G Colony on PDA at day 14. Scale bars: 10 µm (A–E); 10 mm (F, G).