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. 1986 Jan 1;233(1):167-72.
doi: 10.1042/bj2330167.

Characteristics of rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase

G C Ness, C E Sample, M Smith, L C Pendleton, D C Eichler

Characteristics of rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase

G C Ness et al. Biochem J. .

Abstract

A procedure for the preparation of rat liver microsomal fractions essentially devoid of contaminating lysosomes is described. When this preparation was examined by immunoblotting with a rabbit antiserum to rat 3-hydroxy-3-methylglutaryl-CoA reductase, a single band corresponding to an Mr of 100000 was observed. No evidence was found for glycosylation of rat liver-3-hydroxy-3-methylglutaryl-CoA reductase. Native rat liver microsomal 3-hydroxy-3-methylglutaryl-CoA reductase differs from the purified proteolytically modified species in that it displays allosteric kinetics towards NADPH.

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