Aflatoxin B1 (AFB1) production by Aspergillus flavus and Aspergillus parasiticus on ground Nyjer seeds: The effect of water activity and temperature

Abstract

Nyjer oil seed cake supports high levels of aflatoxin B1 (AFB1) production. AFB1 is a secondary metabolite of Aspergillus flavus and A. parasiticus, classified as a Class 1A carcinogen. The aim of this study was to determine the effects of temperature (20, 27, and 35 °C) and water activity (0.82, 0.86, 0.90, 0.94, and 0.98 a_w) on fungal growth and AFB1 production of A. flavus and A. parasiticus on ground Nyjer seeds over a 30-day incubation period. Linear regression models indicated that both fungal growth and AFB1 production were significantly influenced by water activity of Nyjer seeds and incubation temperature. The two fungi did not grow on Nyjer seeds at 0.82 a_w at the three incubation temperatures. The most favorable growth conditions for both fungi were 0.90-0.98 a_w at 27 °C or 0.90-0.94 a_w at 35 °C. The optimum temperature for AFB1 production was 27 °C for both A. flavus and A. parasiticus (with regression coefficients of 6.01 and 9.11, respectively). Both fungi were likely to produce high levels of AFB1 at 0.90 a_w (with regression coefficients of 3.56 for A. flavus and 7.17 for A. parasiticus). Aspergillus flavus only produced AFB1 on seeds with 0.90-0.98 a_w at 27 °C (in the range of 203-282 μg/kg) and on seeds with 0.90 a_w at 35 °C (212 μg/kg). No detectable AFB1 was produced by this fungus in any other culture conditions that were studied. Aspergillus parasiticus, in contrast, was able to produce AFB1 under all of the growth conditions. At 20 °C, this fungus produced the highest level of AFB1 (212 μg/kg) at high water activity (0.98 a_w). At 27 °C, A. parasiticus produced high levels of AFB1 (in the range of 209-265 μg/kg) at a wide range of water activities (0.86-0.98 a_w). In the entire study, the highest AFB1 concertation for A. parasiticus was detected on seeds incubated at high temperature (35 °C) and low water activity (0.86 a_w). The findings of this study could help optimize the storage conditions of Nyjer oil seeds to reduce aflatoxin contamination.

Keywords: Aflatoxin; Aspergillus; Mycotoxin; Nyjer seed; Oil seed.