Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Jan;5(1):51-3.
doi: 10.1002/j.1460-2075.1986.tb04176.x.

Fast calcium transients in rat peritoneal mast cells are not sufficient to trigger exocytosis

E Neher, W Almers

Fast calcium transients in rat peritoneal mast cells are not sufficient to trigger exocytosis

E Neher et al. EMBO J. 1986 Jan.

Abstract

The calcium concentration, [Ca]i, in single rat peritoneal mast cells was measured by means of the new Ca indicator dye fura-2. Upon stimulation with antigen or compound 48/80, [Ca]i rose for seconds to values greater than 5 microM. These Ca transients did not depend on the presence of extracellular Ca, and they sometimes occurred spontaneously, especially in the presence of exogenous phosphatidylserine. Calcium transients did not necessarily lead to degranulation. Degranulation usually occurred during periods of somewhat elevated [Ca] (0.5-1 microM) following transients but was sometimes observed at [Ca]i less than or equal to 250 nM. We found no evidence that an antigen-induced Ca influx is required for degranulation.

PubMed Disclaimer

References

    1. Pflugers Arch. 1981 Aug;391(2):85-100 - PubMed
    1. Proc Natl Acad Sci U S A. 1983 Sep;80(18):5744-8 - PubMed
    1. Nature. 1984 Nov 29-Dec 5;312(5993):453-5 - PubMed
    1. Nature. 1983 Sep 22-28;305(5932):317-9 - PubMed
    1. Nature. 1985 Apr 11-17;314(6011):534-6 - PubMed

Publication types