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. 2019 May;118(5):1581-1592.
doi: 10.1007/s00436-019-06271-4. Epub 2019 Mar 2.

Cathepsin L-a novel cysteine protease from Haemaphysalis flava Neumann, 1897

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Cathepsin L-a novel cysteine protease from Haemaphysalis flava Neumann, 1897

Yali Sun et al. Parasitol Res. 2019 May.

Abstract

Ixodid ticks are ectoparasites responsible for the transmission of a large number of bacterial, viral, and protozoan pathogens to animals and humans. As long-term blood-pool feeders, the digestion of host blood is critical to their development as well as to the establishment of the sexual cycle of hemoparasites such as Babesia parasites, the agents of human and animal babesiosis. Previous studies have demonstrated that cysteine proteases are involved in blood digestion, embryogenesis, and pathogen transmission in other species of ticks, but their characteristics and functions are still unidentified in Haemaphysalis flava. Here, we describe the characterization of a cysteine protease HfCL from H. flava. We show that HfCL belongs to the L-like papain family of proteases, exhibits high expression in nymphs and adults, and localizes to both the midgut and salivary glands. Biochemical assays using purified recombinant enzyme reveal that rHfCL can hydrolyze the fluorogenic substrate Z-phe-Arg-MCA with optimal activity detected at pH 6. Furthermore, the short-term growth assay indicates that rHfCL can inhibit the intraerythrocytic development of Babesia microti and Babesia gibsoni in vitro.

Keywords: Babesia gibsoni; Babesia microti; Cathepsin L; Haemaphysalis flava; Papain.

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References

    1. J Biol Chem. 1999 May 7;274(19):13311-21 - PubMed
    1. Exp Parasitol. 2000 Feb;94(2):75-83 - PubMed
    1. Int J Parasitol. 2000 Nov;30(12-13):1323-37 - PubMed
    1. Dev Biol. 2001 Sep 1;237(1):68-78 - PubMed
    1. Mol Biochem Parasitol. 2002 Mar;120(1):1-21 - PubMed

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