Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Jan 22:14:Doc01.
doi: 10.3205/dgkh000317. eCollection 2019.

Multi-drug resistant Pseudomonas aeruginosa and Klebsiella pneumoniae circulation in a burn hospital, Tehran, Iran

Leila Azimi  1 , Reza Alaghehbandan  2 , Mahla Asadian  3 , Faranak Alinejad  4 , Abdolaziz Rastegar Lari  5
Affiliations

Multi-drug resistant Pseudomonas aeruginosa and Klebsiella pneumoniae circulation in a burn hospital, Tehran, Iran

Leila Azimi et al. GMS Hyg Infect Control. .

Abstract
in English, German

Pseudomonas aeruginosa and Klebsiella pneumoniae are among the most important Gram-negative bacteria that can cause nosocomial infections, especially in burn patients. It is important to determine genetic relationships in different clinical specimens as well as between clinical and environmental specimens, which can aid in detecting the source of infection. The aim of this study was to investigate multi-drug resistant Pseudomonas aeruginosa and Klebsiella pneumoniae spread in a burn hospital, Tehran, Iran. After identification, antibiotic susceptibility testing of all isolates was conducted according to the CLSI guidelines. Further, pulsed-field gel electrophoresis (PFGE) was performed for molecular typing. 97 clinical and 33 environmental specimens were collected. 40 (55%) clinical strains of P. aeruginosa and K. pneumoniae were highly drug resistant. PFGE findings showed similar genetic features to those seen in multi-drug resistant and/or extensively drug resistant P. aeruginosa and K. pneumoniae in clinical and environmental isolates. Inhibition of bacterial spread in the hospital can help to control health care-associated infection and subsequently decrease the morbidity and mortality in hospitalized patients, particularly immunocompromised populations such as burn patients.

Pseudomonas (P.) aeruginosa und Klebsiella (K.) pneumoniae gehören zu den wichtigsten Gram-negativen Erregern nosokomialer Infektionen speziell bei Verbrennungspatienten. Zur Identifizierung der Infektionsquelle ist es wichtig, die genetische Verwandtschaft zwischen Proben des Patienten und aus der patientennahen Umgebung zu bestimmen. Zielsetzung der Studie war die Analyse der Ausbreitung multiresistenter Pseudomonas aeruginosa und Klebsiella pneumoniae Stämme in einem Krankenhaus für Verbrennungspatienten in Teheran. Nach der mikrobiologischen Differenzierung wurde das Antibiogramm gemäß Richtlinie des Clinical Laboratory Standards Institute bestimmt. Die molekulare Typisierung erfolgt mit der Pulsfeld-Gel-Elektrophorese (PFGE). Es wurden 97 Stämme von Patienten und 33 aus der Umgebung analysiert. 40 (55%) Patientenstämme von P. aeruginosa und K. pneumoniae Patientenstämme erwiesen sich als multiresistent bzw. extensiv resistent. Die PFGE zeigte ähnliche genetische Merkmale bei multiresistenten und extensiv resistenten Stämmen beider Species aus klinischen und Umgebungsisolaten. Die Unterbrechung der Erregerausbreitung im Krankenhaus ist eine wichtige Maßnahme zur Prävention nosokomialer Infektionen speziell bei Verbrennungspatienten mit eingeschränkter Immunantwort.

Keywords: K. pneumoniae; P. aeruginosa; antibiotic resistance; genetic relationship; health care-associated infection; pulsed-field gel electrophoresis.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Table 1
Table 1. Genus, frequency and location of environmental isolates
Table 2
Table 2. Antibiotic resistance patterns of both clinical and environmental specimens
Figure 1
Figure 1. PFGE pulsotypes algorithm
A: 1-8; different clinical and environmental isolates of P. aeruginosa B: 1-4; different clinical and environmental isolates of K. pneumoniae
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Azimi L, Motevallian A, Ebrahimzadeh Namvar A, Asghari B, Lari AR. Nosocomial infections in burned patients in motahari hospital, tehran, iran. Dermatol Res Pract. 2011;2011:436952. doi: 10.1155/2011/436952. - DOI - PMC - PubMed
    1. Alaghehbandan R, Azimi L, Rastegar Lari A. Nosocomial infections among burn patients in Teheran, Iran: a decade later. Ann Burns Fire Disasters. 2012 Mar 31;25(1):3–7. - PMC - PubMed
    1. Lari AR, Alaghehbandan R. Nosocomial infections in an Iranian burn care center. Burns. 2000 Dec;26(8):737–740. doi: 10.1016/S0305-4179(00)00048-6. - DOI - PubMed
    1. Zanetti G, Blanc DS, Federli I, Raffoul W, Petignat C, Maravic P, Francioli P, Berger MM. Importation of Acinetobacter baumannii into a burn unit: a recurrent outbreak of infection associated with widespread environmental contamination. Infect Control Hosp Epidemiol. 2007 Jun;28(6):723–725. doi: 10.1086/517956. - DOI - PubMed
    1. Naze F, Jouen E, Randriamahazo RT, Simac C, Laurent P, Blériot A, Chiroleu F, Gagnevin L, Pruvost O, Michault A. Pseudomonas aeruginosa outbreak linked to mineral water bottles in a neonatal intensive care unit: fast typing by use of high-resolution melting analysis of a variable-number tandem-repeat locus. J Clin Microbiol. 2010 Sep;48(9):3146–3152. doi: 10.1128/JCM.00402-10. - DOI - PMC - PubMed