Gonadal microRNA Expression Profiles and Their Potential Role in Sex Differentiation and Gonadal Maturation of Mud Crab Scylla paramamosain

Khor Waiho^{1

2

3}, Hanafiah Fazhan^{1

2}, Yin Zhang^{1

2}, Yueling Zhang^{1

2}, Shengkang Li^{1

2}, Huaiping Zheng^{1

2}, Wenhua Liu^{1

2}, Mhd Ikhwanuddin^{4

5}, Hongyu Ma^{6

7

8}

Affiliations

¹ Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, 515063, China.
² STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou, 515063, China.
³ Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China.
⁴ STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou, 515063, China. ikhwanuddin@umt.edu.my.
⁵ Institute of Tropical Aquaculture, Universiti Malaysia Terengganu, 21030, Kuala Terengganu, Malaysia. ikhwanuddin@umt.edu.my.
⁶ Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, 515063, China. mahy@stu.edu.cn.
⁷ STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou, 515063, China. mahy@stu.edu.cn.
⁸ Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China. mahy@stu.edu.cn.

PMID: 30835008
DOI: 10.1007/s10126-019-09882-1

Gonadal microRNA Expression Profiles and Their Potential Role in Sex Differentiation and Gonadal Maturation of Mud Crab Scylla paramamosain

Khor Waiho et al. Mar Biotechnol (NY). 2019 Jun.

. 2019 Jun;21(3):320-334.

doi: 10.1007/s10126-019-09882-1. Epub 2019 Mar 5.

Authors

Khor Waiho^{1

2

3}, Hanafiah Fazhan^{1

2}, Yin Zhang^{1

2}, Yueling Zhang^{1

2}, Shengkang Li^{1

2}, Huaiping Zheng^{1

2}, Wenhua Liu^{1

2}, Mhd Ikhwanuddin^{4

5}, Hongyu Ma^{6

7

8}

Affiliations

¹ Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, 515063, China.
² STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou, 515063, China.
³ Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China.
⁴ STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou, 515063, China. ikhwanuddin@umt.edu.my.
⁵ Institute of Tropical Aquaculture, Universiti Malaysia Terengganu, 21030, Kuala Terengganu, Malaysia. ikhwanuddin@umt.edu.my.
⁶ Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, 515063, China. mahy@stu.edu.cn.
⁷ STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou, 515063, China. mahy@stu.edu.cn.
⁸ Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China. mahy@stu.edu.cn.

PMID: 30835008
DOI: 10.1007/s10126-019-09882-1

Abstract

Although the sexual dimorphism in terms of gonadal development and gametogenesis of mud crab has been described, the internal regulating mechanism and sex differentiation process remain unclear. A comparative gonadal miRNA transcriptomic study was conducted to identify miRNAs that are differentially expressed between testes and ovaries, and potentially uncover miRNAs that might be involved in sex differentiation and gonadal maturation mechanisms of mud crabs (Scylla paramamosain). A total of 10 known miRNAs and 130 novel miRNAs were identified, among which 54 were differentially expressed. Target gene prediction revealed a significant enrichment in 30 KEGG pathways, including some reproduction-related pathways, e.g. phosphatidylinositol signalling system and inositol phosphate metabolism pathways. Further analysis on six differentially expressed known miRNAs, six differentially expressed novel miRNAs and their reproduction-related putative target genes shows that both miRNAs and putative target genes showed stage-specific expression during gonadal maturation, suggesting their potential regulatory roles in sex differentiation and reproductive development. This study reveals the sex-biased miRNA profile and establishes a solid foundation for understanding the sex differentiation and gonadal maturation mechanisms of S. paramamosain.

Keywords: Gonadal maturation; Mud crab; Scylla paramamosain; Sex differentiation; microRNA.

PubMed Disclaimer

Cited by

Integrative Proteomic and MicroRNA Analysis: Insights Into Mechanisms of Eyestalk Ablation-Induced Ovarian Maturation in the Swimming Crab Portunus trituberculatus.
Meng X, Zhang M, Gao B, Lv J, Li J, Liu P. Meng X, et al. Front Endocrinol (Lausanne). 2020 Aug 14;11:533. doi: 10.3389/fendo.2020.00533. eCollection 2020. Front Endocrinol (Lausanne). 2020. PMID: 32922361 Free PMC article.
Identification and Comparison of microRNAs in the Gonad of the Yellowfin Seabream (Acanthopagrus Latus).
Li S, Lin G, Fang W, Gao D, Huang J, Xie J, Lu J. Li S, et al. Int J Mol Sci. 2020 Aug 8;21(16):5690. doi: 10.3390/ijms21165690. Int J Mol Sci. 2020. PMID: 32784462 Free PMC article.
The miRNAs let-7b and miR-141 Coordinately Regulate Vitellogenesis by Modulating Methyl Farnesoate Degradation in the Swimming Crab Portunus trituberculatus.
Yu X, Zhang M, Liu P, Li J, Gao B, Meng X. Yu X, et al. Int J Mol Sci. 2023 Dec 24;25(1):279. doi: 10.3390/ijms25010279. Int J Mol Sci. 2023. PMID: 38203450 Free PMC article.
Integrated Analysis of mRNA and miRNA Associated with Reproduction in Female and Male Gonads in Abalone (Haliotis discus hannai).
Huang J, Zhou M, She Z, Chen J, Ke C. Huang J, et al. Int J Mol Sci. 2025 Mar 31;26(7):3235. doi: 10.3390/ijms26073235. Int J Mol Sci. 2025. PMID: 40244070 Free PMC article.
Screening of Biomarkers Related to Ovarian Maturation and Spawning in Blunt Snout Bream (Megalobrama amblycephala) Based on Metabolomics and Transcriptomics.
Yi S, Liu LF, Zhou LF, Zhao BW, Wang WM, Gao ZX. Yi S, et al. Mar Biotechnol (NY). 2020 Apr;22(2):180-193. doi: 10.1007/s10126-019-09943-5. Epub 2020 Jan 31. Mar Biotechnol (NY). 2020. PMID: 32006128

See all "Cited by" articles

References

1. Nucleic Acids Res. 2000 Jan 1;28(1):27-30 - PubMed
1. Nat Genet. 2000 May;25(1):25-9 - PubMed
1. Methods. 2001 Dec;25(4):402-8 - PubMed
1. Genetics. 2002 Feb;160(2):537-45 - PubMed
1. Dev Biol. 2003 Jul 1;259(1):9-18 - PubMed

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions

Substances

Actions

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Gonadal microRNA Expression Profiles and Their Potential Role in Sex Differentiation and Gonadal Maturation of Mud Crab Scylla paramamosain

Affiliations

Gonadal microRNA Expression Profiles and Their Potential Role in Sex Differentiation and Gonadal Maturation of Mud Crab Scylla paramamosain

Authors

Affiliations

Abstract

Similar articles

Cited by

References

MeSH terms

Substances

Grants and funding