A comparison of reproductive isolation between two closely related oak species in zones of recent and ancient secondary contact

Abstract

Background: Much of the debate over the evolutionary consequences of hybridization on genetic divergence and speciation results from the breakdown or reinforcement of reproductive barriers in secondary hybrid zones. Among hybrid populations established for different lengths of time following secondary contact, stronger reproductive barriers are generally expected to occur in zones with longer contact. However, in plants no detailed investigation of recent and ancient zones of secondary contact has been conducted despite the importance of such a comparative study. Here, we compare pre- and postzygotic reproductive barriers between two closely related oak species, Quercus mongolica and Q. liaotungensis, in such a situation.

Results: The recorded flowering times of both species overlapped in both contact zones. The fruit set at 10 and 30 days after interspecific hand pollination was not significantly lower than that after intraspecific pollination whenever Q. mongolica or Q. liaotungensis comprised the maternal parents in both populations. These results indicated that neither prezygotic phenological barriers nor interspecific incompatibility could have resulted in the reproductive isolation between the two species in both hybrid zones. However, the proportion of hybrid seeds produced by both species in the ancient zone was significantly lower than that recorded in the recent zone of secondary contact. In addition, the proportion of hybrid seeds simulated to form, assuming both random mating and an absence of postpollination barriers, was significantly higher than that detected in the ancient contact zone but not in the recent contact zone. These results suggest stronger early-acting postzygotic isolation between the two oak species in the ancient relative to the recent contact zone.

Conclusions: Our comparative study demonstrated that postzygotic barriers during seed maturity were the main contributing factor to total reproductive isolation, particularly in the ancient contact zone, which aided species delimitation. In the recently formed secondary contact zone, pre- and postzygotic barriers were not well developed, and a high frequency of natural hybridization was evident. To our knowledge this study provides the first comparison of reproductive isolation between the ancient and recent secondary contact zones in plants and helps to clarify the evolutionary consequences of hybridization in a temporal context.

Keywords: Ancient contact zone; Hybridization; Postzygotic; Quercus; Recent secondary contact; Reproductive isolation; Temporal differentiation.

Fig. 1

Fruit set (mean ± SE) at 10 and 30 days after inter- and intraspecific hand pollination in the ancient and recent secondary contact zone between Quercus liaotungensis and Q. mongolica. The open and solid circles represent fruit set after inter- and intraspecific pollination, respectively. Figures a and b indicate the fruit set at 10 and 30 days after pollination when Q. liaotungensis comprised the maternal plants, respectively, and the Figures c and d indicate the fruit set at 10 and 30 days after pollination when Q. mongolica comprised the maternal plants, respectively

Fig. 2

Proportion of hybrid seeds (mean ± SE) under natural and simulated conditions in populations NA and Dlw. The investigation was conducted in population NA in 2012 (a) and in population Dlw in both 2013 (c) and 2014 (b). The * indicates significant difference in the proportion of hybrid seeds under natural and simulated conditions

Fig. 3

Relationship between tree diameter at breast height (DBH) and the probability of a tree being assigned to Q. liaotungensis in populations NA (a) and Dlw (b). The dashed lines indicate the cut-off value of 0.9. Scatter points above the 0.9 dashed line represent Q. liaotungensis trees, scatter points beneath the 0.1 dashed line represent Q. mongolica trees, and scatter points between the two lines represent the hybrid trees