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. 2019 Mar 6;20(5):1153.
doi: 10.3390/ijms20051153.

Abdominal Fat SIRT6 Expression and Its Relationship with Inflammatory and Metabolic Pathways in Pre-Diabetic Overweight Patients

Nunzia D'Onofrio  1 , Gorizio Pieretti  2 , Feliciano Ciccarelli  3 , Antonio Gambardella  4 , Nicola Passariello  5 , Maria Rosaria Rizzo  6 , Michelangela Barbieri  7 , Raffaele Marfella  8 , Gianfranco Nicoletti  9 , Maria Luisa Balestrieri  10 , Celestino Sardu  11
Affiliations

Abdominal Fat SIRT6 Expression and Its Relationship with Inflammatory and Metabolic Pathways in Pre-Diabetic Overweight Patients

Nunzia D'Onofrio et al. Int J Mol Sci. .

Abstract

: The role of sirtuin 6 (SIRT6) in adipose abdominal tissue of pre-diabetic (pre-DM) patients is poorly known. Here, we evaluated SIRT6 expression in visceral abdominal fat of obese pre-diabetic patients and the potential effects of metformin therapy. Results indicated that obese pre-DM subjects showed low SIRT6 protein expression and high expression of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), peroxisome proliferator-activated receptor gamma (PPAR-γ), and sterol regulatory element-binding transcription factor 1 (SREBP-1). Obese pre-DM patients showed high values of glucose, insulin resistance (HOMA-IR), C reactive protein (CRP), nitrotyrosine, tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6), and low values of insulin (p < 0.05). Of note, abdominal fat tissue of obese pre-DM patients treated with metformin therapy presented higher SIRT6 expression and lower NF-κB, PPAR-γ, and SREBP-1 expression levels compared to pre-DM control group. Collectively, results show that SIRT6 is involved in the inflammatory pathway of subcutaneous abdominal fat of obese pre-DM patients and its expression responds to metformin therapy.

Keywords: NF-κB; PPAR-γ; SREBP-1; obesity; pre-diabetes; sirtuin 6.

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Conflict of interest statement

the authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Protein expression levels of SIRT6, NF-κB, PPAR-γ and SREBP-1 in abdominal adipose tissue. (A) SIRT6, (B) NF-κB, (C) PPAR-γ, and (D) SREBP-1 protein levels determined by Western blot analysis of abdominal adipose tissue homogenates from obese normoglycaemic, obese pre-diabetic patients plus metformin (Met) and obese pre-diabetic patients treated with placebo. Inset, representative images of Western blot analysis. Lane 1, obese normoglycaemic patient; lane 2, obese pre-diabetic+ Met; lane 3, obese pre-diabetic + placebo. Data are mean ± SD. * p < 0.01 vs. obese normoglycemic patients; ‡ p < 0.01 vs. obese pre-diabetic + placebo; p < 0.05 vs. obese pre-diabetic + placebo.
See this image and copyright information in PMC

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