Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Oct-Dec;11(4):383-390.
doi: 10.1016/j.jaim.2018.12.005. Epub 2019 Mar 4.

Cytotoxic and apoptotic inducing activity of Amoora rohituka leaf extracts in human breast cancer cells

Rajesh Kumar Singh  1 , Amit Ranjan  2 , Akhileshwar Kumar Srivastava  3 , Monika Singh  4 , Anil Kumar Shukla  5 , Neelam Atri  6 , Anurag Mishra  7 , Anil Kumar Singh  7 , Santosh Kumar Singh  8
Affiliations

Cytotoxic and apoptotic inducing activity of Amoora rohituka leaf extracts in human breast cancer cells

Rajesh Kumar Singh et al. J Ayurveda Integr Med. 2020 Oct-Dec.

Abstract

Background: Amoora rohituka is described in Ayurveda, an Indian traditional system of medicine for management of disorders of blood, diseases of eye, helminthiasis disease, ulcer, liver disorders and splenomegaly. However, the leaves were not reported to have anticancer properties till date.

Objective: This study was carried out to evaluate the cytotoxic potential of leaf extracts of Amoora rohituka.

Materials and methods: The leaves powder was macerated in petroleum ether, ethyl acetate and methanol and evaluated their anticancer activities in vitro. The phytochemical constituents of the active (ethyl acetate) extract were screened by FTIR analysis and phytochemical screening methods.

Results: The ethyl acetate extract (RLEA) showed the presence of alkaloids, flavonoids, steroids, tannins, saponins and terpenoids. The RLEA exhibited high cytotoxic effect against human breast cancer cells, MCF-7 (IC50 = 9.81 μg/mL) and induced apoptosis by altering nuclear morphology and DNA laddering. Wound healing assays explained the potency of extract to decrease the cell migration.

Conclusion: The extract of Amoora rohituka leaves exhibited anticancer activity with less toxicity and it could be used for development of alternative drugs in the treatment of human breast cancer.

Keywords: Apoptosis; Breast cancer; Cell migration; FTIR; HPTLC; Traditional medicine.

PubMed Disclaimer

Figures

Image 1
Graphical abstract
Fig. 1
Fig. 1
Chemical Fingerprinting of A. rohituka leaf extracts in different organic solvents. Solvent System: (Toluene: Acetone: Ethyl acetate: 7.5: 2.0: 0.5); Track 1 = Petroleum Ether Extract, Track 2 = Ethyl acetate and Track 3 = Methanolic Extract; and Infrared Spectroscopy.
Fig. 2
Fig. 2
Describing the cytotoxicity of different extracts of A. rohituka leaves with their respective IC50 values (PE: Petroleum ether extract; EA: Ethyl acetate extract; M: Methanol extract).
Fig. 3
Fig. 3
Microscopy of the cells showing alteration in their cellular and nuclear morphology in the treated group (A: treated with packlitaxel; B: treated with Amoora rohituka leave extract, RLEA; C: without any treatment, control negative) and also DNA laddering in the treated group (A: treated with packlitaxel; B: treated with RLEA; C: without any treatment, control negative) in agarose gel which supporting the induction of apoptosis by the extract.
Fig. 4
Fig. 4
Wound healing showing inhibition of cellular migration in treated group of MCF-7 cells at the concentration of 0.78 μg/mL ethyl acetate extract of A. rohituka leaves (RLEA).
See this image and copyright information in PMC

References

    1. Arya R.K., Singh A., Yadav N.K., Cheruvu S.H., Hossain Z., Meena S., et al. Anti-breast tumor activity of Eclipta extract in-vitro and in-vivo: novel evidence of endoplasmic reticulum specific localization of Hsp60 during apoptosis. Sci Rep. 2015;5:18457. doi: 10.1038/srep18457. - DOI - PMC - PubMed
    1. International Agency for Research on Cancer: GLOBOCAN: Estimated cancer incidence, mortality and prevalence worldwide in 2012. Cancer fact sheets. http://globocan.iarc.fr/Pages/fact_sheets_cancer.aspx.
    1. Patwardhan B., Vaidya A.D.B., Chorghade M. Ayurveda and natural products drug discovery. Curr Sci. 2004;86:789–799.
    1. Cragg G.M., Grothaus P.G., Newman D.J. Impact of natural products on developing new Anti-cancer agents. Chem Rev. 2009;109:3012–3043. doi: 10.1021/cr900019j. - DOI - PubMed
    1. Sharma S.B., Gupta R. Drug development from natural Resource: a systematic approach. Mini Rev Med Chem. 2015;15:52–57. - PubMed