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. 2019 Jan;48(1):156-161.

Candiduria in Hospitalized Patients and Identification of Isolated Candida Species by Morphological and Molecular Methods in Ilam, Iran

Afagh Fazeli  1 , Parivash Kordbacheh  1 , Ali Nazari  2 , Roshanak Daie Ghazvini  1 , Hossein Mirhendi  1 , Mahin Safara  1 , Heidar Bakhshi  1 , Razieh Yaghoubi  1
Affiliations

Candiduria in Hospitalized Patients and Identification of Isolated Candida Species by Morphological and Molecular Methods in Ilam, Iran

Afagh Fazeli et al. Iran J Public Health. 2019 Jan.

Abstract

Background: Candiduria in hospitalized patients may represent contamination, colonization, or urinary tract infections. On the other hand, candidemia and upper urinary tract infection could be the complications of candiduria. The aim of this study was to determine candiduria in hospitalized patients and identify isolated Candida species by conventional and molecular methods.

Methods: This cross-sectional study was conducted on hospitalized patients in Imam Khomeini and Mostafa Khomeini hospitals in Ilam, western Iran from Jan to Dec 2016. Urine samples of hospitalized patients were collected during a period of 4 months for diagnosis of candiduria. Primary identification was done by conventional methods. PCR profile was carried out using phenol-chloroform method and confirmed using restriction fragment length polymorphism (PCR-RFLP) technique by MspI restriction enzyme.

Results: Candiduria was diagnosed in 18 (9.2%) cases from a total of 195 patients. Isolated yeasts were identified as C. albicans (n: 13), C. glabrata (n: 5), and C. parapsilosis (n: 1) in the one case both C. albicans and C. glabrata were isolated from a urine sample.

Conclusion: Candida urinary tract infection is becoming increasingly common in hospitalized patients but, differentiation fungal colonization from infection and identification of etiologic agents for optimal treatment is necessary.

Keywords: Candida albicans; Candida spp.; Candiduria; Hospitalized patients; Yeast.

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Conflict of interest statement

Conflict of interest The authors declare that there is no conflict of interests.

Figures

Fig. 1:
Fig. 1:
Candida albicans isolated from urine culture on CHROMagar candida incubated at 35 °C for 48 h
Fig. 2:
Fig. 2:
Candida glabrata isolated from urine culture on CHROM agar candida incubated at 35 °C for 48
Fig. 3:
Fig. 3:
Candida parapsilosis isolated from urine culture onCHROM agar candida incubated at 35 °C for 48
Fig. 4:
Fig. 4:
Candida glabrata and C. albicans isolated together from urine culture on CHROM agar candida incubated at 35 °C for 48
Fig. 5:
Fig. 5:
Budding yeast cells, pseudohyphae and true mycelium in direct examination of urine sediment (×400)
Fig. 6:
Fig. 6:
Patterns of PCR-RFLP products of Candida spp. isolated from candiduria after digestion by the restriction enzyme MspI. Lanes of 1,2,3,4,7,8,9,13,14 represent C. albicans (238, 297 bp); 5,6,10,11,12 C. glabrata (557, 314 bp) and 15 C. parapsilosis (520 bp). Lane M is 100 bp ladder molecular size marker
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