[Selection of appropriate ribonuclease inhibitors in the study of polysomes in human lymphocytes]

Abstract

Polysome profiles are described for lymphocytes of donor peripheral blood treated with various inhibitors of RNAases. The most effective inhibitor was diethyl pyrocarbonate, which enabled to maintain high content of polysomes (up to 70%) in these cells. Ribosomes, treated with diethyl pyrocarbonate, exhibited functional activity in the puromycin test. A natural inhibitor of RNAases from liver tissue inhibited incompletely the enzymatic activity in lymphocytes. Heparin proved to be an inadequate inhibitor of RNAases in all the cells of lymphoid origin (lymphocytes from peripheral blood of donors and of the patients with chronic lympholeukosis, lymphocytes isolated from adenoids and spleen tissue); it induced disaggregation of polysomes and dissociation of ribosomes.