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Comparative Study
. 1986 Apr;154(1):282-6.
doi: 10.1016/0003-2697(86)90527-0.

A fluorometric assay for biotinidase

H Ebrahim, K Dakshinamurti
Comparative Study

A fluorometric assay for biotinidase

H Ebrahim et al. Anal Biochem. 1986 Apr.

Abstract

An assay for biotinidase using biocytin, the natural substrate, is described. The fluorometric procedure uses 1,2-diacetylbenzene which reacts selectively with lysine allowing its direct determination in mixture with biocytin. We have examined the applicability of the assay using human serum biotinidase.

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