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. 1986 Apr;10(4):231-8.
doi: 10.1016/0309-1651(86)90069-x.

Immunoelectronmicroscopic localization of actin in ionophore-treated boar sperm

M Camatini, A Casale, G Anelli, M Cifarelli

Immunoelectronmicroscopic localization of actin in ionophore-treated boar sperm

M Camatini et al. Cell Biol Int Rep. 1986 Apr.

Abstract

Actin in ionophore-A23187 treated boar sperm has been localized by indirect immunofluorescence and immunoelectronmicroscopy (IEM), using an anti-actin monoclonal antibody. By IEM, after en bloc staining technique and treatment with 15 nm colloidal gold-IgM complex, actin was found associated with the plasma membrane (PM) and the outer acrosomal membrane vesicles and under the PM of the equatorial segment.

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