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. 2019 Feb 26:10:300.
doi: 10.3389/fmicb.2019.00300. eCollection 2019.

Probiotic Properties of Enterococcus Isolated From Artisanal Dairy Products

Yousef Nami  1 , Reza Vaseghi Bakhshayesh  1 , Hossein Mohammadzadeh Jalaly  1 , Hajie Lotfi  1 , Solat Eslami  1   2 , Mohammad Amin Hejazi  1
Affiliations

Probiotic Properties of Enterococcus Isolated From Artisanal Dairy Products

Yousef Nami et al. Front Microbiol. .

Abstract

The present study focused on probiotic characterization and safety evaluation of Enterococcus isolates from different artisanal dairy products. All the isolates exhibited inhibitory activity against several food spoilage bacteria and food-borne pathogens, including Shigella flexneri, Staphylococcus aureus, Listeria monocytogenes, Yersinia enterocolitica, Klebsiella pneumoniae, Escherichia coli, and Bacillus subtilis. The PCR results indicated the presence of at least one enterocin structural gene in all the tested strains. The Enterococcus isolates were further evaluated regarding their safety properties and functional features. The isolates were susceptible to vancomycin, gentamycin, and chloramphenicol. The results of PCR amplification revealed that all the tested isolates harbored none of the tested virulence genes except E. faecalis (ES9), which showed the presence of esp gene. The Enterococcus isolates showed cholesterol lowering properties. The selected isolates showed a high tolerance to low pH, and toward bile salts. They also demonstrated hydrophobicity activity, auto-aggregation, and adhesion ability to the human intestinal Caco-2 cell line. These properties may contribute the bacteria colonizing the gut. This study revealed that the Enterococcus isolates, especially E. durans ES11, ES20 and ES32, might be excellent candidates for production of functional foods to promote health benefits.

Keywords: Enterococcus; Enterococcus as probiotics; antimicrobial activity; dairy products; low cholesterol; probiotic properties; safety evaluation; virulence factors.

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Figures

FIGURE 1
FIGURE 1
The hydrophobicity, auto-aggregation and adhesion ability of strains to human intestinal cells. a-gMeans in the same color with different lowercase letters differed significantly (p < 0.05).
FIGURE 2
FIGURE 2
The analysis of the phylogeny of the isolated Enterococcus strains alignment of the sequences was performed with the sequences of different Enterococcus and Lactobacillus species which were submitted in NCBI database as complete sequence.
See this image and copyright information in PMC

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