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Case Reports
. 2019 Apr;9(4):136.
doi: 10.1007/s13205-019-1639-2. Epub 2019 Mar 11.

BaCf3: highly thermostable bacteriocin from Bacillus amyloliquefaciens BTSS3 antagonistic on food-borne pathogens

E S Bindiya  1 , K J Tina  1 , Raghul Subin Sasidharan  1 , Sarita G Bhat  1
Affiliations
Case Reports

BaCf3: highly thermostable bacteriocin from Bacillus amyloliquefaciens BTSS3 antagonistic on food-borne pathogens

E S Bindiya et al. 3 Biotech. 2019 Apr.

Abstract

In the present study, we characterized bacteriocin BaCf3, isolated and purified from Bacillus amyloliquefaciens BTSS3, and demonstrated its inhibitory potential on growth and biofilm formation of certain food spoilage bacteria and pathogens. Purification was by gel filtration chromatography and its molecular weight was 3028.422 Da after MALDI-TOF MS. The bacteriocin was highly thermostable withstanding even autoclaving conditions and pH tolerant (2.0-13.0). The bacteriocin was sensitive to oxidizing agent (DMSO) and reducing agent (DTT). The de novo sequence of the bacteriocin BaCf3 was identified and was found to be novel. The sequence analysis shows the presence of a disulphide linkage between C6 and C13. The microtitre plate assay proved that BaCf3 could reduce up to 80% biofilm produced by strong biofilm producers from food samples. In addition, BaCf3 did not show cytotoxicity on 3-TL3 cell line.

Keywords: Bacillus; Bacteriocin; Biofilm; Cytotoxicity; MALDI-TOF.

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Conflict of interest statement

Compliance with ethical standardsThe authors have no conflict of interest.

Figures

Fig. 1
Fig. 1
MALDI-TOF MS of BaCf3. Inset is the SDS PAGE and in-gel activity of BaCf3. Lane a: Purified BaCf3, Lane b: Low Range Peptide marker (GeNei). Lane c: In-gel activity analysis
Fig. 2
Fig. 2
Effect of a temperature b pH and c metal ions on BaCf3. The activity was calculated by twofold dilution method hence the decrease in values is in a stepwise response (twofold reduction in activity)
Fig. 3
Fig. 3
a Inhibitory action of BaCf3 on food-borne bacteria. b Inhibitory action of BaCf3 on food pathogens. c Growth inhibition of 3-TL3 cell line by different concentrations of BaCf3
See this image and copyright information in PMC

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