doi: 10.1007/978-1-4939-9148-8_6.
Sample Preparation of Trypanosoma cruzi Surface, Extracellular, and Nuclear Subproteomes
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- PMID: 30868520
- DOI: 10.1007/978-1-4939-9148-8_6
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Sample Preparation of Trypanosoma cruzi Surface, Extracellular, and Nuclear Subproteomes
Methods Mol Biol.
2019.
Abstract
The preparation of subproteome fractions prior to proteome analysis provides both the enrichment of proteins sub-represented in global proteome analysis and information on the cellular localization of the identified proteins. Here we describe protocols for the preparation of Trypanosoma cruzi surface and extracellular and nuclear fractions for further subproteome analysis.
Keywords: Biotinylation; Cell surface shaving; Nucleus; Plasma membrane; Proteome; Secretome.
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