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. 2019 Mar 14;14(3):e0213989.
doi: 10.1371/journal.pone.0213989. eCollection 2019.

Expression of Concern: Autophagosome-Mediated EGFR Down-Regulation Induced by the CK2 Inhibitor Enhances the Efficacy of EGFR-TKI on EGFR-Mutant Lung Cancer Cells with Resistance by T790M

PLOS ONE Editors

Expression of Concern: Autophagosome-Mediated EGFR Down-Regulation Induced by the CK2 Inhibitor Enhances the Efficacy of EGFR-TKI on EGFR-Mutant Lung Cancer Cells with Resistance by T790M

PLOS ONE Editors. PLoS One. .
No abstract available

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Figures

Fig 1
Fig 1. CX-4945 induced the autophagy in EGFR-TKI-resistant PC-9 cells.
A, Cells were treated with different concentrations of CX-4945 for 72 h, and the rate of inhibition was determined by MTT assay. B, Cells were treated with or without CX-4945 (5 μM) for 48 h and were then incubated for 24 h with a drug-free medium containing 10% FBS. Pictures showing the autophagic vacuole formation (AVOs) were taken at ×20 magnification. C and D, Cells were treated with CX-4945 for 48 h. Cell lysates were subjected to Western blot analysis. Quantitative detection of acidic vesicular organelles by acridine orange staining of cells was determined by FACS analysis. *p<0.01 and **p<0.001 compared with the control. E, Cells were transfected with a plasmid to express LC3-GFP. After 24 h transfection, cells were treated with CX-4945 (5 μM) for 24 h. Punctate pattern of LC3 localization analyzed by immunofluorescence microscopy. F and G, Cells were incubated with CX-4945 (5 μM), 17-DMAG (100 nM) or rapamycin (20 μM) for 48 h. Pictures were taken at ×20 magnification. The induction of LC3-I/II was shown by Western blot analysis.
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Expression of concern for

References

    1. So KS, Kim CH, Rho JK, Kim SY, Choi YJ, Song JS, et al. (2014) Autophagosome-Mediated EGFR Down-Regulation Induced by the CK2 Inhibitor Enhances the Efficacy of EGFR-TKI on EGFR-Mutant Lung Cancer Cells with Resistance by T790M. PLoS ONE 9(12): e114000 10.1371/journal.pone.0114000 - DOI - PMC - PubMed

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