Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Mar 14;9(1):4477.
doi: 10.1038/s41598-019-41101-8.

Serum levels of miR-126 and miR-223 and outcomes in chronic kidney disease patients

Ophélie Fourdinier #  1   2 , Eva Schepers #  3 , Valérie Metzinger-Le Meuth  1   4 , Griet Glorieux  3 , Sophie Liabeuf  1   5 , Francis Verbeke  3 , Raymond Vanholder  3 , Benjamin Brigant  1   6 , Anneleen Pletinck  3 , Momar Diouf  7 , Stéphane Burtey  8 , Gabriel Choukroun  1   2 , Ziad A Massy  9 , Laurent Metzinger  10   11 ; European Uremic Toxin Work Group-EUTox
Collaborators, Affiliations

Serum levels of miR-126 and miR-223 and outcomes in chronic kidney disease patients

Ophélie Fourdinier et al. Sci Rep. .

Abstract

Several microRNAs (miRNAs) have been linked to chronic kidney disease (CKD) mortality, cardiovascular (CV) complications and kidney disease progression. However, their association with clinical outcomes remains poorly evaluated. We used real-time qPCR to measure serum levels of miR-126 and miR-223 in a large cohort of 601 CKD patients (CKD stage G1 to G5 patients or on renal replacement therapy - CKD G5D) from Ghent University Hospital and 31 healthy controls. All-cause mortality and cardiovascular and renal events were registered as endpoints over a 6 year follow-up period. miR-126 levels were significantly lower from CKD stage G2 on, compared to controls. The serum levels of miR-223 were significantly lower from CKD stage G3B on. When considering overall mortality, patients with levels of either miR-126 or miR-223 below the median had a lower survival rate. Similar results were observed for CV and renal events. The observed link between the two miRNAs' seric levels and mortality, cardiovascular events or renal events in CKD appears to depend on eGFR. However, this does not preclude their potential role in the pathophysiology of CKD. In conclusion, CKD is associated with a decrease in circulating miR-223 and miR-126 levels.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Study flow chart.
Figure 2
Figure 2
Relative expression (serum levels) of miRNAs in healthy controls, patients at different CKD stages, and patients on RRT. (A) Serum levels of miR-223. (B) Serum levels of miR-126. Data are shown as the median with box and whisker plots, including the 10th and 90th percentiles. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (in a Mann-Whitney test).
Figure 3
Figure 3
Unadjusted survival curves for primary outcomes for participants with above-median and below-median serum miR-223 (A and B) or miR-126 (C and D) levels (Log-rank test).
Figure 4
Figure 4
Unadjusted survival curves for secondary outcomes for participants with above-median and below-median serum miR-223 (A and B) or miR-126 (C and D) levels (Log-rank test).
See this image and copyright information in PMC

References

    1. Vallianou NG, Mitesh S, Gkogkou A, Geladari E. Chronic Kidney Disease and Cardiovascular Disease: is there any relationship? Curr Cardiol Rev. Curr Cardiol Rev. 2019;15(1):55. - PMC - PubMed
    1. Rennenberg RJ, et al. Vascular calcifications as a marker of increased cardiovascular risk: a meta-analysis. Vasc Health Risk Manag. 2009;5(1):185. - PMC - PubMed
    1. Rong D, et al. An emerging function of circRNA-miRNAs-mRNA axis in human diseases. Oncotarget. 2017;8(42):73271. - PMC - PubMed
    1. Lee RC, Feinbaum RL, Ambros V. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell. 1993;75(5):843. - PubMed
    1. Metzinger-Le Meuth V, et al. The expanding roles of microRNAs in kidney pathophysiology. Nephrol Dial Transplant. 2019;34(1):7. - PubMed

Publication types