Fluorescent Polymer Dots for Tracking SKOV₃ Cells in Living Mice with Probe-Based Confocal Laser Endomicroscopy

Yufan Zhang¹, Yao Li¹, Yixiao Guo¹, Yidian Yang^{1

2}, Shiyi Tang¹, Liqin Xiong³

Affiliations

¹ Shanghai Med-X Engineering Center for Medical Equipment and Technology, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, People's Republic of China.
² The Key Laboratory of Resource Chemistry of Ministry of Education, Shanghai Key Laboratory of Rare Earth Functional Materials, and Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, Shanghai Normal University, Shanghai, 200234, People's Republic of China.
³ Shanghai Med-X Engineering Center for Medical Equipment and Technology, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, People's Republic of China. xiongliqin@sjtu.edu.cn.

PMID: 30877592
DOI: 10.1007/s11307-019-01343-4

Fluorescent Polymer Dots for Tracking SKOV₃ Cells in Living Mice with Probe-Based Confocal Laser Endomicroscopy

Yufan Zhang et al. Mol Imaging Biol. 2019 Dec.

. 2019 Dec;21(6):1026-1033.

doi: 10.1007/s11307-019-01343-4.

Authors

Yufan Zhang¹, Yao Li¹, Yixiao Guo¹, Yidian Yang^{1

2}, Shiyi Tang¹, Liqin Xiong³

Affiliations

¹ Shanghai Med-X Engineering Center for Medical Equipment and Technology, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, People's Republic of China.
² The Key Laboratory of Resource Chemistry of Ministry of Education, Shanghai Key Laboratory of Rare Earth Functional Materials, and Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, Shanghai Normal University, Shanghai, 200234, People's Republic of China.
³ Shanghai Med-X Engineering Center for Medical Equipment and Technology, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, People's Republic of China. xiongliqin@sjtu.edu.cn.

PMID: 30877592
DOI: 10.1007/s11307-019-01343-4

Abstract

Purpose: Probe-based confocal laser endomicroscopy (pCLE) is a novel technique allowing real-time and high-resolution imaging in vivo. It provides microscopic images and increases the penetration depth of tissues compared with conventional white light endoscopy. The aim of the present study was to track ovarian cancer cells in organs by fluorescent polymer dots based on pCLE.

Procedures: SKOV₃-mCherry cells were incubated with polymer dots for 24 h in a serum-free culture medium. Labeled cells were administrated to nude mice via intravenous, intraperitoneal, and lymph node injection. The fluorescent signals of labeled cells in organs were observed by pCLE. Furthermore, the results were confirmed by frozen section analysis.

Results: pCLE displayed fluorescence signals of labeled cells in the vessels of organs. Besides, the accumulations of labeled cells visualized in detoxification organs like the spleen and kidney were increased with time.

Conclusions: In this article, we present a real-time and convenient method for tracking SKOV₃-mCherry in living mice by combined fluorescent polymer dots with pCLE.

Keywords: Fluorescence imaging; Ovarian cancer; Polymer dots; Tracking; pCLE.

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Fluorescent Polymer Dots for Tracking SKOV₃ Cells in Living Mice with Probe-Based Confocal Laser Endomicroscopy

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Fluorescent Polymer Dots for Tracking SKOV₃ Cells in Living Mice with Probe-Based Confocal Laser Endomicroscopy

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