Structures of the Catalytically Activated Yeast Spliceosome Reveal the Mechanism of Branching

Abstract

Pre-mRNA splicing is executed by the spliceosome. Structural characterization of the catalytically activated complex (B^∗) is pivotal for understanding the branching reaction. In this study, we assembled the B^∗ complexes on two different pre-mRNAs from Saccharomyces cerevisiae and determined the cryo-EM structures of four distinct B^∗ complexes at overall resolutions of 2.9-3.8 Å. The duplex between U2 small nuclear RNA (snRNA) and the branch point sequence (BPS) is discretely away from the 5'-splice site (5'SS) in the three B^∗ complexes that are devoid of the step I splicing factors Yju2 and Cwc25. Recruitment of Yju2 into the active site brings the U2/BPS duplex into the vicinity of 5'SS, with the BPS nucleophile positioned 4 Å away from the catalytic metal M2. This analysis reveals the functional mechanism of Yju2 and Cwc25 in branching. These structures on different pre-mRNAs reveal substrate-specific conformations of the spliceosome in a major functional state.

Keywords: ATPase/helicases; B(∗) complex; RNA splicing; branch point sequence; branching reaction; catalytically activated spliceosome; cryo-EM structure; splicosome; step I factors; substrate-specific.