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. 2019 Apr;25(4):780-783.
doi: 10.3201/eid2504.180812.

Genomic Survey of Bordetella pertussis Diversity, United States, 2000-2013

Michael R Weigand, Margaret M Williams, Yanhui Peng, Dane Kania, Lucia C Pawloski, Maria L Tondella; CDC Pertussis Working Group
Collaborators

Genomic Survey of Bordetella pertussis Diversity, United States, 2000-2013

Michael R Weigand et al. Emerg Infect Dis. 2019 Apr.

Abstract

We characterized 170 complete genome assemblies from clinical Bordetella pertussis isolates representing geographic and temporal diversity in the United States. These data capture genotypic shifts, including increased pertactin deficiency, occurring amid the current pertussis disease resurgence and provide a foundation for needed research to direct future public health control strategies.

Keywords: Bordetella pertussis; United States; bacteria; genomics; pertussis; vaccines; whooping cough.

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Figures

Figure 1
Figure 1
Bordetella pertussis diversity, United States, 2000–2013. A) Geographic origin of B. pertussis isolates selected to maximize the number of source states from each of 6 time periods. Pie chart diameter represents the number of isolates, as detailed in the key, and colors indicate time periods, as shown in panel B. B) Isolate frequency by time period. C) Relative abundance of MLST types prn2-ptxP3-ptxA1-fimH1 (gray), prn2-ptxP3-ptxA1-fimH2 (white), and other (black). Red line indicates frequency of pertactin-deficient alleles. D) Abundance distribution of genome structures. Black bar indicates unique structures (singletons) and gray bars the 16 colinear groups. MLST, multilocus sequence typing.
Figure 2
Figure 2
Phylogenetic reconstruction of all 170 isolates and the reference Tohama I (GenBank accession no. CP010964). Isolate metadata and molecular characteristics are color coded, as detailed in the key. Scale bar indicates substitutions per site. CDC, Centers for Disease Control and Prevention; fim, fimbria; fwd, forward insertion; rev, reverse insertion; PFGE, pulsed-field gel electrophoresis; prn, pertactin; ptx, pertussis toxin.
See this image and copyright information in PMC

References

    1. Clark TA. Changing pertussis epidemiology: everything old is new again. J Infect Dis. 2014;209:978–81. 10.1093/infdis/jiu001 - DOI - PubMed
    1. Ausiello CM, Cassone A. Acellular pertussis vaccines and pertussis resurgence: revise or replace? MBio. 2014;5:e01339–14. 10.1128/mBio.01339-14 - DOI - PMC - PubMed
    1. Pawloski LC, Queenan AM, Cassiday PK, Lynch AS, Harrison MJ, Shang W, et al. Prevalence and molecular characterization of pertactin-deficient Bordetella pertussis in the United States. Clin Vaccine Immunol. 2014;21:119–25. 10.1128/CVI.00717-13 - DOI - PMC - PubMed
    1. Martin SW, Pawloski L, Williams M, Weening K, DeBolt C, Qin X, et al. Pertactin-negative Bordetella pertussis strains: evidence for a possible selective advantage. Clin Infect Dis. 2015;60:223–7. 10.1093/cid/ciu788 - DOI - PubMed
    1. Breakwell L, Kelso P, Finley C, Schoenfeld S, Goode B, Misegades LK, et al. Pertussis vaccine effectiveness in the setting of pertactin-deficient pertussis. Pediatrics. 2016;137:e20153973. 10.1542/peds.2015-3973 - DOI - PubMed

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