Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Mar 18;7(1):43.
doi: 10.1186/s40478-019-0692-8.

Tumor cell-free DNA detection in CSF for primary CNS lymphoma diagnosis

V Rimelen  1 , G Ahle  2 , E Pencreach  3 , N Zinniger  4 , A Debliquis  4 , L Zalmaï  4 , I Harzallah  4 , R Hurstel  5 , I Alamome  5 , F Lamy  2 , J Voirin  2 , B Drénou  4
Affiliations

Tumor cell-free DNA detection in CSF for primary CNS lymphoma diagnosis

V Rimelen et al. Acta Neuropathol Commun. .
No abstract available

PubMed Disclaimer

Conflict of interest statement

Ethics approval and consent to participate

All patients had given written informed consent. An institutional review board had approved the study, which was conducted in accordance with the declaration of Helsinki.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Figures

Fig. 1
Fig. 1
MYD88 L265P quantification by ddPCR. Technical workflow for the CSF analysis and 2D ddPCR diagram of the fluorescence amplitude. Lower left quadrant contains the droplets with no MYD88 alleles; upper left contains droplets with MYD88L265P cfDNA; upper right contains droplets with both wild-type and mutant alleles; lower right contains droplets with MYD88 wild-type DNA. ddPCR results table for cell and cell-free CSF and brain biopsy, comparison with cytology and FCM. NA, not available; ND, not determined; NI, not interpretable
See this image and copyright information in PMC

References

    1. Baraniskin A, Chomiak M, Ahle G, Gress T, Buchholz M, Turewicz M, Eisenacher M, Margold M, Schlegel U, Schmiegel W, Hahn S, Schroers R. MicroRNA-30c as a novel diagnostic biomarker for primary and secondary B-cell lymphoma of the CNS. J Neuro-Oncol. 2018;137:463–468. doi: 10.1007/s11060-018-2749-0. - DOI - PubMed
    1. Bonzheim I, Giese S, Deuter C, Süsskind D, Zierhut M, Waizel M, Szurman P, Federmann B, Schmidt J, Quintanilla-Martinez L, Coupland SE, Bartz-Schmidt KU, Fend F. High frequency of MYD88 mutations in vitreoretinal B-cell lymphoma: a valuable tool to improve diagnostic yield of vitreous aspirates. Blood. 2015;126:76–79. doi: 10.1182/blood-2015-01-620518. - DOI - PubMed
    1. Chapuy B, Stewart C, Dunford AJ, Kim J, Kamburov A, Redd RA, Lawrence MS, Roemer MGM, Li AJ, Ziepert M, Staiger AM, Wala JA, Ducar MD, Leshchiner I, Rheinbay E, Taylor-Weiner A, Coughlin CA, Hess JM, Pedamallu CS, Livitz D, Rosebrock D, Rosenberg M, Tracy AA, Horn H, van Hummelen P, Feldman AL, Link BK, Novak AJ, Cerhan JR, Habermann TM, Siebert R, Rosenwald A, Thorner AR, Meyerson ML, Golub TR, Beroukhim R, Wulf GG, Ott G, Rodig SJ, Monti S, Neuberg DS, Loeffler M, Pfreundschuh M, Trümper L, Getz G, Shipp MA. Molecular subtypes of diffuse large B cell lymphoma are associated with distinct pathogenic mechanisms and outcomes. Nat Med. 2018;24:679–690. doi: 10.1038/s41591-018-0016-8. - DOI - PMC - PubMed
    1. Debliquis A, Voirin J, Harzallah I, Maurer M, Lerintiu F, Drénou B, Ahle G. Cytomorphology and flow cytometry of brain biopsy rinse fluid enables faster and multidisciplinary diagnosis of large B-cell lymphoma of the central nervous system. Cytometry B Clin Cytom. 2018;94:182–188. doi: 10.1002/cyto.b.21403. - DOI - PubMed
    1. Fontanilles M, Marguet F, Bohers É, Viailly P-J, Dubois S, Bertrand P, Camus V, Mareschal S, Ruminy P, Maingonnat C, Lepretre S, Veresezan E-L, Derrey S, Tilly H, Picquenot J-M, Laquerrière A, Jardin F. Non-invasive detection of somatic mutations using next-generation sequencing in primary central nervous system lymphoma. Oncotarget. 2017;8:48157–48168. doi: 10.18632/oncotarget.18325. - DOI - PMC - PubMed