Comparative Study
doi: 10.1093/nar/14.12.4731.
In vitro transcription of bacteriophage phi 29 DNA. Correlation between in vitro and in vivo promoters
- PMID: 3088543
- PMCID: PMC311487
- DOI: 10.1093/nar/14.12.4731
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Comparative Study
In vitro transcription of bacteriophage phi 29 DNA. Correlation between in vitro and in vivo promoters
Nucleic Acids Res.
.
Free PMC article
Abstract
The phi 29 DNA in vitro transcription initiation sites have been accurately mapped by S1 protection experiments. The results obtained indicated that the B. subtilis RNA polymerase containing the sigma 43 subunit basically recognized the same set of phi 29 promoters in vitro as those used in vivo. In addition, the sequence of the phi 29 early A2a promoter used both in vitro and in vivo has been determined as well as the precise nucleotide where initiation of transcription from the C2 promoter occurs in vitro.
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