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. 2019 Feb 25:6:222-231.
doi: 10.1016/j.toxrep.2019.02.009. eCollection 2019.

Mainstream smoke constituents and in vitro toxicity comparative analysis of 3R4F and 1R6F reference cigarettes

Guy Jaccard  1 , Donatien Tafin Djoko  1 , Alexandra Korneliou  1 , Regina Stabbert  1 , Maxim Belushkin  1 , Marco Esposito  1
Affiliations

Mainstream smoke constituents and in vitro toxicity comparative analysis of 3R4F and 1R6F reference cigarettes

Guy Jaccard et al. Toxicol Rep. .

Erratum in

Abstract

A new Kentucky reference cigarette, 1R6F, has been manufactured to replace the depleting 3R4F reference cigarette. The 3R4F Kentucky reference cigarettes have been widely used as monitor or comparator cigarettes for mainstream smoke analysis and in vitro and in vivo toxicological data of cigarettes and novel tobacco products. Both reference cigarettes were analyzed in the same laboratory during the same period of time with the goal of performing a comparison of 3R4F and 1R6F. On the basis of the results obtained from aerosol chemistry and in vitro assays, we consider that the 1R6F reference cigarette is a suitable replacement for the 3R4F reference cigarette as a comparator/monitor cigarette. Its specific use as a comparator for novel tobacco products was checked on the basis of a comparative test with the Tobacco Heating System 2.2 as an example.

Keywords: Cigarettes in vitro toxicity; Cigarettes mainstream smoke; Reference cigarettes.

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Figures

None
Graphical abstract
Fig. 1
Fig. 1
Graphical representation of the percentage difference between the yields of measured constituents in MS obtained under the ISO smoking regime from 1R6F and 3R4F reference cigarettes.
Fig. 2
Fig. 2
Graphical representation of the percentage difference between the yields of measured constituents in MS obtained under the ISO Intense smoking regime from 1R6F and 3R4F reference cigarettes.
Fig. 3
Fig. 3
Relative % difference between Ames assay-specific activity slopes for TPM from MS of 1R6F and 3R4F cigarettes obtained under ISO (green circles) and ISO Intense (orange triangles) smoking conditions related to the long-term variability of the 3R4F (critical difference, CD).
Fig. 4
Fig. 4
Relative % difference between IC50 for TPM and GVP from MS of 1R6F and 3R4F cigarettes obtained under ISO (green circles) and ISO Intense (orange triangles) smoking conditions related to the long-term variability of the 3R4F (critical difference, CD).
Fig. 5
Fig. 5
Relative % difference between ivMN linear regression slopes [number of MN/(mg TPM/mL)] for TPM from MS of 1R6F and 3R4F cigarettes obtained under ISO (green circles) and ISO Intense (orange triangles) smoking conditions compared to the long-term variability of the 3R4F (critical difference, CD) for each treatment schedule.
Fig. 6
Fig. 6
Reduction of HPHC yields for THS 2.2 in comparison with 3R4F (blue) and 1R6F (orange) cigarettes, using the ISO Intense analytical smoking machine regime.
See this image and copyright information in PMC

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