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. 2019 Feb 10;9(5):2706-2714.
doi: 10.1002/ece3.4941. eCollection 2019 Mar.

Structure and genetic variability of golden mussel (Limnoperna fortunei) populations from Brazilian reservoirs

Pâmela Juliana Furlan-Murari  1 , Claudete de Fatima Ruas  2 , Eduardo Augusto Ruas  2 , Lucas Milanez Benício  2 , Angela Maria Urrea-Rojas  1 , Angela Rocio Poveda-Parra  1 , Emerson Murari  3 , Ed Christian Suzuki de Lima  1 , Felipe Pinheiro de Souza  1 , Nelson Mauricio Lopera-Barrero  1
Affiliations

Structure and genetic variability of golden mussel (Limnoperna fortunei) populations from Brazilian reservoirs

Pâmela Juliana Furlan-Murari et al. Ecol Evol. .

Abstract

The golden mussel, Limnoperna fortunei a highly invasive species in Brazil, has generated productive, economical, and biological impacts. To evaluate genetic structure and variability of L. fortunei populations present in fish farms in the reservoirs of Canoas I (CANFF), Rosana (ROSFF), and Capivara (CAPFF) (Paranapanema River, Paraná, Brazil), eight microsatellite loci were amplified. Five of those eight loci resulted in 38 alleles. The observed heterozygosity (Ho) was lower than the expected heterozygosity (He) in all populations, with a deviation from the Hardy-Weinberg equilibrium (HWE). The average value for the inbreeding coefficient (Fis) was positive and significative for all populations. There was higher genetic variability within populations than among them. The fixation index (Fst) showed a small genetic variability among these populations. The occurrence of gene flow was identified in all populations, along with the lack of a recent bottleneck effect. The clustering analysis yielded K = 2, with genetic similarity between the three populations. The results demonstrate low genetic structure and suggest a founding population with greater genetic variability (ROSFF). Our data point to the possible dispersal of L. fortunei aided by anthropic factors in the upstream direction. It was concluded that the three populations presented a unique genetic pool for Paranapanema River, with occurrence of gene flow.

Keywords: bioinvader; bivalve; environmental conservation; genetic analysis; microsatellite markers.

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Conflict of interest statement

The authors reported no potential conflict of interest.

Figures

Figure 1
Figure 1
Location of the Canoas I (1), Capivara (2), and Rosana (3) reservoirs, indicated by the points, and the CANFF collection sites (22°56′25.63″S; 50°24′49.86″ W), CAPFF (22°41′17.16″S; 51°17′51.30″W), and ROSFF (22°39′25.20″S; 52°46′52.78″W), indicated by the stars, along the Paranapanema River
Figure 2
Figure 2
(a) Graphic representation of the first two factorial correspondence analysis (FCA), blue squares—ROSFF, white squares—CAPFF, and yellow squares—CANFF; (b) Clustering analysis of (Limnoperna fortunei) from Brazilian reservoirs
See this image and copyright information in PMC

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