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. 2017 Mar;12(1):1-6.
doi: 10.1016/j.jds.2016.03.013. Epub 2016 May 13.

Apical extrusion of Enterococcus faecalis in different canal geometries during the use of nickel titanium systems with different motion types

Ugur Aydin  1 , Yasemin Zer  2 , Mehtap Zorlu Golge  1 , Esra Kirkgoz Karabulut  2 , Emre Culha  3 , Emrah Karataslioglu  1
Affiliations

Apical extrusion of Enterococcus faecalis in different canal geometries during the use of nickel titanium systems with different motion types

Ugur Aydin et al. J Dent Sci. 2017 Mar.

Abstract

Background/purpose: Extrusion of intracanal bacteria leads to treatment failures. Compare the apical extrusion of intracanal bacteria (Enterococcus faecalis) during canal preparation with three different instrumentation techniques [RECIPROC, One Shape (OS), and Twisted-File Adaptive (TFA)] with different motion types.

Materials and methods: Ninety teeth with different canal morphologies were divided into three main groups, each including 30 teeth (10 mandibular incisors, 10 mandibular premolars, and 10 curved roots). Roots were resected until 13-mm working length was obtained and fixed to glass vials filled with brain-heart infusion broth. Each canal was filled with E. faecalis suspension. The three main groups were further grouped into three subgroups. Each group was further subgrouped into three, with each subgroup including 10 roots from each type of teeth (10 incisors/subgroup, 10 premolars/subgroup, and 10 curved canals/subgroup). These subgroups were prepared with one of RECIPROC, OS, or TFA. Bacterial colonies extruded into each vial were incubated in brain-heart infusion agar at 37°C for 5 days and counted using a colony counter as the number of colony-forming units per milliliter. Statistical analyses were performed using one-way analysis of variance, post hoc Tukey honest significant difference, and Kruskal-Wallis tests.

Results: Apically extruded bacteria were not statistically different from each other (P > 0.05). The amount of apically extruded bacteria was statistically similar for both different instruments in the same type of tooth (P > 0.05) and same instrument in different types of teeth (P > 0.05).

Conclusion: Neither the motion type of instrument nor the canal morphology affected the degree of bacterial extrusion.

Keywords: Enterococcus faecalis; One Shape; RECIPROC; Twisted-File Adaptive; bacterial extrusion.

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Figures

Figure 1
Figure 1
A sample embedded and fixed into a glass vial filled with brain–heart infusion broth through the rubber stopper of the vial. Needle was inserted into the rubber stopper to equalize the pressure.
Figure 2
Figure 2
A sample of the test apparatus.
Figure 3
Figure 3
One of microscopic images of the positive control group.
Figure 4
Figure 4
Flowchart of the experiment.
See this image and copyright information in PMC

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