Correction: Trail Resistance Induces Epithelial-Mesenchymal Transition and Enhances Invasiveness by Suppressing PTEN via miR-221 in Breast Cancer

Abstract

[This corrects the article DOI: 10.1371/journal.pone.0099067.].

Fig 4. Down-regulation of PTEN resulted in resistance to TRAIL of 231 cells, EMT and enhancement of invasiveness.

A. PTEN was down-regulated in 231T cells compared with 231. B. MDA-MB-231 cells transfected with siPTEN and its corresponding NC were named as 231-siPTEN and 231-n respectively. Tolerance of 231-n cells and 231-siPTEN cells to different concentrations of TRAIL was examined by MTT assay. Points represented the average of three independent experiments. Bars stood for SD; C. Migration and invasion assay of 231-n cells and 231-siPTEN cells. Count the cells in 10 representative fields under a light microscope. D. N-cadherin, fibronectin, vimentin and Snail of 231-n and 231-siPTEN cells were detected by western blot assay. Also b-actin was used as control. E. Summary graphs for migration and invasion, respectively. Data were shown as mean 6 SD. *P,0.05; **P,0.01.

Fig 6. PTEN was the target gene of miR-221.

A. miR-221 was predicted to regulate PTEN. B. After silencing miR-221, the mRNA level of PTEN was significantly up-regulated detected by real-time PCR. C. The corresponding change of PTEN in 231T-n and 231T-si221 cells was detected by western blot assay. *P,0.05; **P,0.01.

Fig 7. MiR-221 knockdown could sensitize 231T cells to TRAIL, reverse EMT and reduce cell mobility.

A. Cultured 231T-n and 231Tsi221 cells with different concentrations of TRAIL in 96-well plates. 48 hours later examined their sensitivity to TRAIL by MTT assay. Points represented the average of three independent experiments. Bars stood for SD; B. The mesenchymal markers, N-cadherin, fibronectin, vimentin and Snail expressed in 231T-n and 231T-si221 cells were detected by western blot assay.b-actin was used as control; C. Migration and invasion assay of 231T-n cells and 231T-si221 cells. Staining the migrated cells with hematoxylin–eosin and count them in 10 representative fields under a light microscope. D. Summary graphs for migration and invasion were also shown, respectively. Data was presented as mean6SD. *P,0.05; **P,0.01.