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. 2019 Feb:167:41-45.
doi: 10.1016/j.jcpa.2018.12.002. Epub 2019 Jan 14.

An Outbreak of Fatal Bordetella bronchiseptica Bronchopneumonia in Puppies

J K Chambers  1 , I Matsumoto  1 , T Shibahara  2 , M Haritani  3 , H Nakayama  1 , K Uchida  4
Affiliations

An Outbreak of Fatal Bordetella bronchiseptica Bronchopneumonia in Puppies

J K Chambers et al. J Comp Pathol. 2019 Feb.

Abstract

Twenty-two newborn puppies that did not receive colostrum exhibited acute respiratory signs and died at a breeding facility. Pathological examinations were performed on four of the puppies. At necropsy examination, the lungs were firm and mottled dark red, consistent with acute bronchopneumonia. Histopathologically, there was marked infiltration of neutrophils and macrophages into the bronchi and alveoli, and gram-negative coccobacilli were attached diffusely to the cilia of bronchial mucosa. Immunohistochemistry for Bordetella bronchiseptica antigen revealed positive labelling of the bacterial agents. On electron microscopy, a large number of coccobacilli were observed attaching to the cilia of bronchial epithelial cells. Real-time polymerase chain reaction amplified a B. bronchiseptica gene from the affected lung tissue. Based on these findings, the four puppies were diagnosed with fatal B. bronchiseptica bronchopneumonia.

Keywords: Bordetella bronchiseptica; bronchopneumonia; colostrum; dog.

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Figures

Fig. 1
Fig. 1
Pathological findings of B. bronchiseptica bronchopneumonia in puppies. (A) The lung is diffusely swollen with dark red areas. Bar, 1 cm. (B) Severe infiltration of neutrophils and macrophages into the bronchi and alveoli. HE. Bar, 200 μm. (C) Basophilic bacteria on the surface of bronchial epithelium. HE. Bar, 50 μm. (D) Large numbers of bacteria are attached to the cilia of bronchial epithelial cells. HE. Bar. 20 μm. (E) IHC for B. bronchiseptica antigen. Bacteria on the surface of the bronchial epithelium are positively labelled. Positive signals are also seen in the cytoplasm of inflammatory cells in the bronchus. Bar, 50 μm. (F) IHC for B. bronchiseptica antigen. Numerous positive signals in the alveolus with severe inflammation. Bar, 50 μm.
Fig. 2
Fig. 2
Electron microscopy of B. bronchiseptica bronchopneumonia in puppies. (A) SEM of the bronchial mucosa. Numerous coccobacilli are attached to the cilia of bronchial mucosa. Bar, 10 μm. (B) TEM of the bronchial mucosa. Coccobacilli with membranous pili attached to the cilia of bronchial epithelial cells. Bar, 1 μm.
See this image and copyright information in PMC

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