The Expression and Clinical Significance of Spleen Tyrosine Kinase in Patients with Coronary Heart Disease

Abstract

BACKGROUND Our research was designed to investigate the relationship of spleen tyrosine kinase (Syk) and inflammatory factors with coronary heart disease (CHD) and the risk factors of CHD. MATERIAL AND METHODS In our study, 226 patients were enrolled, from October 2017 to March 2018. Clinical and biochemical data were collected. We collected samples of peripheral blood monocytes (PBMs) from the enrolled patients. The patients were divided in 4 groups: patients without coronary artery disease (control group), patients with stable angina pectoris (SAP group), patients with non-ST-segment elevation acute coronary syndrome (NSTE-ACS group), and patients with ST-segment elevation acute myocardial infarction group (STEMI group). We detect the protein levels of Syk and inflammatory factors expression by western blot. RESULTS Our results found the protein levels of Syk and inflammatory factors expression in the NSTE-ACS and STEMI groups were higher than those in the SAP and control groups. The protein levels of Syk and inflammatory factors expression in the SAP group were higher than those in the control group. Moreover, there were many risk factors significantly associated with Syk. Besides that, these risk factors were also independent risk factors of CHD. CONCLUSIONS Our results found that the level of Syk was associated with the severity of CHD. From our study, we found that higher levels of Syk and inflammatory factors protein were associated with worse results of the CHD. For the first time, Syk was reported to be a promising therapeutic factor for CHD patients.

Figure 1

The protein expression levels of Syk, IL-6, IL-12, MMP-9 and MCP-1 in the peripheral blood monocytes of the 4 groups of patients by western blotting. Peripheral blood monocytes were obtained from patients with STEMI, NSTEACS, SAP, and control groups. (A) The protein expression levels of Syk in the peripheral blood monocytes of the 4 groups were analyzed by western blotting and quantitative. (B) The protein expression levels of IL-6 in the peripheral blood monocytes of the 4 groups were analyzed by western blotting and quantitative. (C) The protein expression levels of IL-12 in the peripheral blood monocytes of the 4 groups were analyzed by western blotting and quantitative. (D) The protein expression levels of MMP-9 in the peripheral blood monocytes of the four groups were analyzed by western blotting and quantitative. (E) The protein expression levels of MCP-1 in the peripheral blood monocytes of the 4 groups were analyzed by western blotting and quantitative. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001 compared to control. The quantitative data were expressed as the mean ±SEM of at least 3 independent experiments. Control – patients without coronary stenosis; NSTE-ACS – non-ST-segment elevation acute coronary syndrome; SAP – stable angina pectoris; STEMI – ST segment elevation myocardial infarction.

Figure 2

Analysis of the correlation between Syk and inflammatory factors. (A) There is a positive correlation between Syk and IL-6 in patients with or without coronary heart disease (CHD). (B) There is a positive correlation between Syk and IL-12 in patients with or without CHD. (C) There is a positive correlation between Syk and MMP-9 in patients with or without CHD. (D) There is a positive correlation between Syk and MCP-1 in patients with or without CHD.