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. 2019 Mar;15(2):416-423.
doi: 10.5114/aoms.2018.76928. Epub 2018 Jul 5.

Expression of tryptophan hydroxylase in gastric mucosa in symptomatic and asymptomatic Helicobacter pylori infection

Cezary Chojnacki  1 , Tomasz Popławski  2 , Aleksandra Błońska  1 , Janusz Błasiak  2 , Marek Romanowski  3 , Jan Chojnacki  1
Affiliations

Expression of tryptophan hydroxylase in gastric mucosa in symptomatic and asymptomatic Helicobacter pylori infection

Cezary Chojnacki et al. Arch Med Sci. 2019 Mar.

Abstract

Introduction: Helicobacter pylori infection induces clinical symptoms in 15-20% of subjects, and the reason for this variation is still not clear. The aim of the present study is to evaluate the expression of tryptophan hydroxylase (TpH-1) in gastric mucosa of patients with symptomatic and asymptomatic H. pylori infection in relation to the intensity of bacterial colonization and severity of dyspeptic symptoms.

Material and methods: Ninety subjects (aged 35-49 years) were enrolled in the study and separated into 3 groups of 30 subjects each. Group I - healthy volunteers without H. pylori infection, group II - subjects with asymptomatic H. pylori infection, group III - H. pylori-infected patients with dyspeptic symptoms, mainly fasting and nocturnal epigastric pain. To diagnose H. pylori infection the urea breath test (UBT-13C) and histological analysis were performed. The level of mRNA expression of tryptophan hydroxylase (TpH-1) was estimated in gastric mucosa with RT-PCR.

Results: The expression of this enzyme in antral mucosa was 2.69 ±0.97 in group I, 2.28 ±0.69 in group II (p > 0.05) and 4.40 ±1.64 in group III (p < 0.001). The levels of expression of TpH-1 in gastric body mucosa were 2.16 ±0.70, 1.57 ±0.52 (p > 0.05) and 3.40 ±1.51 (p < 0.001), respectively. In group III a positive correlation was found between intensity of H. pylori colonization and TpH-1 expression as well as between TpH-1 expression and severity of dyspeptic symptoms.

Conclusions: Increased expression of TpH-1 in gastric mucosa plays a role in pathogenesis of chronic dyspepsia.

Keywords: Helicobacter pylori; chronic dyspepsia; gastric mucosa; tryptophan hydroxylase.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Expression of tryptophan hydroxylase (TpH-1) in antral gastric mucosa in healthy volunteers (group I), subjects with asymptomatic (group II) and those with symptomatic (group III) Helicobacter pylori infection. Statistical differences: I vs. II p > 0.05; I vs. III p < 0.001; II vs. III p < 0.001
Figure 2
Figure 2
Expression of tryptophan hydroxylase (TpH-1) in body gastric mucosa in healthy volunteers (group I), subjects with asymptomatic (group II) and those with symptomatic (group III) Helicobacter pylori infection. Statistical differences: I vs. II p > 0.05; I vs. III p < 0.001; II vs. III p < 0.001
Figure 3
Figure 3
Correlation between intensity of Helicobacter pylori infection (13C-UBT) and expression of tryptophan hydroxylase (TpH-1) in antral gastric mucosa; r = 0.575, p < 0.05
Figure 4
Figure 4
Correlation between intensity of Helicobacter pylori infection (13C-UBT) and expression of tryptophan hydroxylase (TpH-1) in body gastric mucosa; r = 0.4993, p > 0.05
Figure 5
Figure 5
Correlation between expression of tryptophan hydroxylase (TpH-1) in antral gastric mucosa and severity of dyspeptic symptoms; r = 0.8549, p < 0.01
Figure 6
Figure 6
Correlation between expression of tryptophan hydroxylase (TpH-1) in antral gastric mucosa and severity of dyspeptic symptoms; r = 0.8019, p < 0.001
Figure 7
Figure 7
Expression of tryptophan hydroxylase (TpH-1) in antral (A) and body (B) gastric mucosa, before (A1, B1) and after (A2, B2) eradication of Helicobacter pylori. Differences in both parts of gaster are statistically significant; A1 vs. A2 p < 0.01; B1 vs. B2 p < 0.001
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