Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Mar;15(2):416-423.
doi: 10.5114/aoms.2018.76928. Epub 2018 Jul 5.

Expression of tryptophan hydroxylase in gastric mucosa in symptomatic and asymptomatic Helicobacter pylori infection

Cezary Chojnacki  1 , Tomasz Popławski  2 , Aleksandra Błońska  1 , Janusz Błasiak  2 , Marek Romanowski  3 , Jan Chojnacki  1
Affiliations

Expression of tryptophan hydroxylase in gastric mucosa in symptomatic and asymptomatic Helicobacter pylori infection

Cezary Chojnacki et al. Arch Med Sci. 2019 Mar.

Abstract

Introduction: Helicobacter pylori infection induces clinical symptoms in 15-20% of subjects, and the reason for this variation is still not clear. The aim of the present study is to evaluate the expression of tryptophan hydroxylase (TpH-1) in gastric mucosa of patients with symptomatic and asymptomatic H. pylori infection in relation to the intensity of bacterial colonization and severity of dyspeptic symptoms.

Material and methods: Ninety subjects (aged 35-49 years) were enrolled in the study and separated into 3 groups of 30 subjects each. Group I - healthy volunteers without H. pylori infection, group II - subjects with asymptomatic H. pylori infection, group III - H. pylori-infected patients with dyspeptic symptoms, mainly fasting and nocturnal epigastric pain. To diagnose H. pylori infection the urea breath test (UBT-13C) and histological analysis were performed. The level of mRNA expression of tryptophan hydroxylase (TpH-1) was estimated in gastric mucosa with RT-PCR.

Results: The expression of this enzyme in antral mucosa was 2.69 ±0.97 in group I, 2.28 ±0.69 in group II (p > 0.05) and 4.40 ±1.64 in group III (p < 0.001). The levels of expression of TpH-1 in gastric body mucosa were 2.16 ±0.70, 1.57 ±0.52 (p > 0.05) and 3.40 ±1.51 (p < 0.001), respectively. In group III a positive correlation was found between intensity of H. pylori colonization and TpH-1 expression as well as between TpH-1 expression and severity of dyspeptic symptoms.

Conclusions: Increased expression of TpH-1 in gastric mucosa plays a role in pathogenesis of chronic dyspepsia.

Keywords: Helicobacter pylori; chronic dyspepsia; gastric mucosa; tryptophan hydroxylase.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Expression of tryptophan hydroxylase (TpH-1) in antral gastric mucosa in healthy volunteers (group I), subjects with asymptomatic (group II) and those with symptomatic (group III) Helicobacter pylori infection. Statistical differences: I vs. II p > 0.05; I vs. III p < 0.001; II vs. III p < 0.001
Figure 2
Figure 2
Expression of tryptophan hydroxylase (TpH-1) in body gastric mucosa in healthy volunteers (group I), subjects with asymptomatic (group II) and those with symptomatic (group III) Helicobacter pylori infection. Statistical differences: I vs. II p > 0.05; I vs. III p < 0.001; II vs. III p < 0.001
Figure 3
Figure 3
Correlation between intensity of Helicobacter pylori infection (13C-UBT) and expression of tryptophan hydroxylase (TpH-1) in antral gastric mucosa; r = 0.575, p < 0.05
Figure 4
Figure 4
Correlation between intensity of Helicobacter pylori infection (13C-UBT) and expression of tryptophan hydroxylase (TpH-1) in body gastric mucosa; r = 0.4993, p > 0.05
Figure 5
Figure 5
Correlation between expression of tryptophan hydroxylase (TpH-1) in antral gastric mucosa and severity of dyspeptic symptoms; r = 0.8549, p < 0.01
Figure 6
Figure 6
Correlation between expression of tryptophan hydroxylase (TpH-1) in antral gastric mucosa and severity of dyspeptic symptoms; r = 0.8019, p < 0.001
Figure 7
Figure 7
Expression of tryptophan hydroxylase (TpH-1) in antral (A) and body (B) gastric mucosa, before (A1, B1) and after (A2, B2) eradication of Helicobacter pylori. Differences in both parts of gaster are statistically significant; A1 vs. A2 p < 0.01; B1 vs. B2 p < 0.001
See this image and copyright information in PMC

References

    1. Biernat MM, Poniewierka E, Błaszczuk J, et al. Antimicrobial susceptibility of Helicobacter pylori isolates from Lower Silesia, Poland. Arch Med Sci. 2014;10:505–9. - PMC - PubMed
    1. Matusiak M, Chałubiński M, Broncel M, et al. Putative consequences of exposure to Helicobacter pylori infection in patients with coronary heart disease in terms of humoral immune response and inflammation. Arch Med Sci. 2016;12:45–54. - PMC - PubMed
    1. Jais M, Barua S. Seroprevalence of anti Helicobacter pylori IgG/IgA in asymptomatic population from Delhi. J Commun Dis. 2004;36:132–5. - PubMed
    1. Dinić M, Tasić G, Stanković-Dordević D, Otasević L, Tasić M, Karanikolic A. Serum anti-Helicobacter pylori IgA and IgG antibodies in asymptomatic children in Serbia. Scand J Infect Dis. 2007;39:303–7. - PubMed
    1. Sumona AA, Hossain MA, Musa AK, et al. Anti H. pylori IgM in symptomatic and asymptomatic population. Mymensingh Med J. 2009;18:18–20. - PubMed