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. 2019 Mar 22;14(3):e0213993.
doi: 10.1371/journal.pone.0213993. eCollection 2019.

Multilocation comparison of fruit composition for 'HoneySweet', an RNAi based plum pox virus resistant plum

Ann M Callahan  1 , Chris D Dardick  1 , Ralph Scorza  1
Affiliations

Multilocation comparison of fruit composition for 'HoneySweet', an RNAi based plum pox virus resistant plum

Ann M Callahan et al. PLoS One. .

Abstract

'HoneySweet', a transgenic plum (Prunus domestica) resistant to plum pox virus through RNAi, was deregulated in the U.S. in 2011. The compositional study of 'HoneySweet' fruit was expanded to include locations outside of the US as well as utilizing a wide variety of comparators and different collection years to see the variability possible. The results revealed that plums have a wide variation in composition and that variation among locations was greater than variation among cultivars. This was also the case for different years at one location. The results supported the supposition that the transgene and insertion event had no significant effect on the composition of 'HoneySweet' fruit even under virus pressure, and that it fell in the normal range of composition of commercially grown plums. It also suggested that the effect of environment is as great as that of genetics on the fruit composition of plums.

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Conflict of interest statement

No competing interests are declared. ‘HoneySweet’ plum is patented (USPP15154P2, 2004) jointly by Institut National de la Recherche Agronomique (INRA), Cornell Research Foundation Inc and the US Department of Agriculture with Ralph Scorza listed as one of the inventors. It is freely available. Ralph Scorza LLC has no competing interests. This does not alter our adherence to PLOS ONE policies on sharing data and materials. (as detailed online in our guide for authors http://journals.plos.org/plosone/s/competing-interests) as all materials and data are freely available.

Figures

Fig 1
Fig 1. Composite of all the plums used in the composition analyses.
Picture of the fruit as it was received. ‘HoneySweet’ picture is from previous fruit.
Fig 2
Fig 2. Distribution of values for individual fruit samples for four components.
The three multilocation samples, ‘HoneySweet’ in blue, ‘Stanley’ in grey, ‘Jojo’ in orange and the other 20 cultivars in yellow, are graphed for Titratable Acid, Quinic Acid, Vitamin C and Fructose. ‘HoneySweet’ is consistently high for titratable acid, and ‘Stanley’ and ‘Jojo’ are consistently low for quinic acid. All the others overlap.
Fig 3
Fig 3. Effects by location.
Titratable acid is again consistently high in ‘HoneySweet’ regardless of the location. In contrast, the vitamin C level is affected by location. Where it is consistently high in the southern Spain location for ‘HoneySweet’ as well as two cultivars in Germany. The box plot graphes further show the effect using averages and high and low points. CZ-Czech Republic, US-United States, ESs-southern Spain, ESn-northern Spain, BG-Bulgaria, IT-Italy, PL-Poland, RS-Serbia, RO-Romania, CA-Canada, DE-Germany, FR-France, HS-‘HoneySweet’, JJ-‘Jojo’, ST-‘Stanley’.
See this image and copyright information in PMC

References

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